circSNTB2和CUL4A 通过具有竞争性结合性的miR-66565诱导细胞核的功能障碍
Yiming Jia1, Xin Huo2, Leilei Wu1
1Department of Spine Surgery, Chifeng Municipal Hospital, Hongshan District, No. 1, Zhaowuda Road, Chifeng City, 024000, Inner Mongolia, China.
Biochemical genetics
|July 28, 2023
概括
循环RNA circSNTB2通过调节miR-665和CUL4A.促进腰椎椎间板 (LDH). 向circSNTB2可能通过控制细胞核核细胞增殖和细胞亡来为LDH提供一种新的治疗策略.
科学领域:
- 分子生物学分子生物学
- 生物化学 生化学
- 再生医学是一种再生医学.
背景情况:
- 循环RNAs (circRNAs) 与各种疾病有关,包括腰部退行性疾病.
- 椎间盘退化 (IDD) 和腰椎间盘 (LDH) 在脊柱健康方面构成重大挑战.
- circSNTB2在LDH病变发生中的特定作用仍然在很大程度上未被探索.
研究的目的:
- 研究circSNTB2在腰椎间盘 (LDH) 发生中的作用.
- 阐明circSNTB2影响细胞核脉 (NP) 细胞行为的分子机制.
- 评估circSNTB2作为LDH的潜在治疗点.
主要方法:
- 生物信息学分析确定了IDD中的circSNTB2表达.
- 在体外研究中,利用TNF-α治疗的NP细胞来模仿LDH,通过CCK-8,EdU,TUNEL和流细胞计评估增殖和亡.
- 在体内实验中建立了一个LDH大鼠模型来验证研究结果.
主要成果:
- 在LDH患者的组织和TNF-α治疗的NP细胞中,CircSNTB2显著上调.
- 通过miR-665/CUL4A轴的介导,CircSNTB2的淘汰促进了NP细胞的增殖和抑制了细胞灭亡.
- 在体内,circSNTB2敲击减轻了LDH诱导的疼痛和NP损伤.
结论:
- 通过miR-665/CUL4A通路调节NP细胞增殖和细胞亡,CircSNTB2促进LDH.
- CircSNTB2代表了LDH的一个有前途的治疗标.
- 针对circSNTB2/miR-665/CUL4A轴为LDH治疗提供了一个新的策略.
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