马诺阿利德通过氧化应激和线粒体功能障碍在人类骨髓瘤细胞中诱导内在亡
Zhi-Kang Yao1,2, Yen-Hsuan Jean3, Sung-Chun Lin3
1Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 80424, Taiwan.
Antioxidants (Basel, Switzerland)
|July 29, 2023
概括
马诺利德是一种海洋海绵化合物,通过诱导氧化应激和线粒体损伤,有效地杀死骨髓瘤细胞. 这种天然化合物显示出作为这种侵袭性骨癌的新治疗方法的希望.
科学领域:
- 生物化学 生物化学
- 在瘤学瘤学.
- 海洋天然产品 化学 化学
背景情况:
- 骨髓瘤 (OS) 是一种主要的恶性骨瘤,死亡率高,特别是在转移性病例中.
- 马诺阿利德是一种来自海洋海绵的sesterterpenoid,具有抗炎,止痛和抗癌的特性.
- 了解manoalide对OS的机制对于开发新的治疗策略至关重要.
研究的目的:
- 为了研究manoalide对人类骨髓瘤细胞 (143B和MG63) 的细胞毒性作用和潜在机制.
- 阐明manoalide对氧化应激,线粒体功能和OS细胞的亡途径的影响.
主要方法:
- 对143B和MG63细胞进行24小时和48小时的治疗,使用不同度的manoalide (10,20,40μM).
- 评估细胞活力,活性氧物种 (ROS) 水平,抗氧化蛋白表达和亡标记物 (caspase-9/-3, PARP).
- 评估线粒体功能,包括氧化酸化,ATP生成,膜潜力和线粒体动力学 (融合/裂变蛋白).
主要成果:
- 马诺阿利德显著降低了MG63细胞活力,这种活力取决于剂量和时间.
- 马诺阿利德诱导了ROS的过度生产,破坏了抗氧化蛋白,并激活了亡.
- 曼诺利德通过影响氧化酸化,ATP生成,膜潜力和促进线粒体分裂,损害了线粒体功能.
结论:
- 马诺阿利德通过氧化应激,线粒体功能障碍和亡诱导骨髓瘤细胞死亡.
- N-乙-l-氨酸的预处理减轻了马诺阿利德诱导的毒性,证实了ROS的作用.
- 马诺阿利德显示出作为人类骨髓瘤的创新治疗剂的潜力.
关键词:
抗氧化剂 抗氧化剂是一种抗氧化剂.灭症 (apoptosis) 是一种死亡的过程.这是一种炎症炎症炎症炎症.线粒体中的线粒体.线粒体呼吸链是线粒体的呼吸链.骨质肉瘤是骨质肉瘤的一种疾病.氧化应激是一种氧化应激.有活性氧物种的反应性氧物种.更多相关视频
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