对细菌结合和抗生素的天然替代品的干扰:弥合差距
Micaela Guidotti-Takeuchi1, Roberta Torres de Melo1, Lígia Nunes de Morais Ribeiro2
1Laboratory of Molecular Epidemiology, Federal University of Uberlândia, Uberlândia 38402-018, MG, Brazil.
Antibiotics (Basel, Switzerland)
|July 29, 2023
概括
纳米结构的脂质纳米载体 (NLCs) 含有香和香精油,显著降低了在食品矩阵中沙门氏菌和大肠杆菌之间的抗菌素耐药性基因转移. 这些NLC为控制工业环境中的病原体进化提供了一个有希望的策略.
科学领域:
- 食品微生物学 食品微生物学
- 抗微生物耐药性 抗微生物耐药性
- 基因转移是基因的转移.
背景情况:
- 横向基因转移 (HGT) 驱动了食物传播病原体中抗菌素耐药性 (AMR) 的演变.
- 了解食品矩阵中的HGT对于控制公共健康风险至关重要.
- 黑德堡沙门氏菌 (SH) 和大肠杆菌 (EC) 是主要的食物传播病原体.
研究的目的:
- 在工业食品条件下研究SH和EC之间的HGT.
- 评估纳米结构脂质纳米载体 (NLCs) 与精油的抗结合疗效.
- 为了确定食品副产品 (乳清和汁) 对HGT的影响.
主要方法:
- 鉴定S.海德堡的抗生素耐药性和blaTEM基因的存在.
- 结合频率的评估 (CF) 有或没有NLCs.
- 在0.39 mg/mL时,对来自圣和香精油的NLCs进行评估.
- 使用乳清 (SL) 和汁 (CJ) 作为食品矩阵.
主要成果:
- 食品副产品 (SL和CJ) 分别增加了 3.5 和 2.5 个数量级的结合 (p < 0.01).
- 与对照组相比,精油NLCs降低了CF的74.90-124.4倍.
- 橄香精油NLCs根据条件降低了CF的4.46-11.3倍.
结论:
- 含有香和香精油的NLC有效地减轻了抗生素耐药基因的HGT.
- 这些NLC代表了控制工业食品环境中的AMR的有希望的战略.
- 有针对性的NLC应用可以减少食物传播病原体中耐药性基因的传播.
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