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关于素DD14的审查,这是一种无领导的两细菌素,具有多种生物功能和不寻常的运输途径

Rabia Ladjouzi¹, Elodie Dussert¹, Radja Teiar¹

¹UMR Transfrontalière BioEcoAgro, INRAe 1158, ICV-Institut Charles Viollette, University Lille, INRAE, University Liège, UPJV, YNCREA, University Artois, University Littoral Côte d'Opale, F-59000 Lille, France.

Antibiotics (Basel, Switzerland)

|July 29, 2023

概括

素DD14是一种来自人类菌 (Enterococcus faecalis) 的细菌素,具有广泛的抗微生物和抗病毒活性. 它还增强了抗生素对MRSA的疗效,并减少了炎症,表明了治疗潜力.

科学领域:

微生物学微生物学
细菌素研究研究细菌素研究
治疗剂开发治疗剂开发

背景情况:

菌素DD14 (EntDD14) 是一种新型的双无细菌素 (LLB),由人类开始性菌株*Enterococcus faecalis*14产生.
细菌素是通过核糖体合成的抗微生物,在医学和生物技术中具有潜在的应用.

研究的目的:

描述EntDD14.的抗微生物,抗病毒和免疫调节特性.
调查EntDD14生产的遗传基础和运输机制.
探索EntDD14的治疗潜力,特别是与抗生素结合使用.

主要方法:

抗微生物和抗病毒活性测定对各种格拉姆阳性病原体和细胞系.
使用像Bagel4.4这样的生物信息学工具对*E. faecalis* 14的基因组测序和注释.
使用小鼠模型进行体外和体外研究,以评估EntDD14对MRSA抗生素活性的增强.
在人类肠道细胞中对促炎性细胞因子分泌的分析.

主要成果:

EntDD14对*Listeria monocytogenes*,*Clostridium perfringens*,*Enterococcus faecalis*和*Staphylococcus aureus*表现出强烈的活性,并且可以对抗这些病毒.
在体外和体内,EntDD14显著增强了红色素,干amycin 和甲素对MRSA的疗效.
EntDD14表现出抗病毒性质,并降低了Caco-2细胞中促炎性IL-6和IL-8的分泌.
确定了EntDD14集群,并且证明其运输涉及PH域蛋白质 (DdE/DdF) 和ABC传送器 (DdGHIJ) 的复杂系统.

结论:

EntDD14是一种有前途的多功能细菌素,具有显著的抗微生物,抗病毒和免疫调节作用.
独特的运输机制突出了细菌素出口和自我保护的新战略.
EntDD14代表了研究双LLB的有价值模型,并具有治疗应用的潜力,包括对抗抗生素耐药细菌的打击.

关键词:

氨酸DD14 (EntDD14) 的使用方法它具有抗菌和抗病毒的特性.没有领导的细菌素 (LLB)特定的运输特定的运输.

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