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锁定了:Phoenixin-14不会穿过由干细胞衍生的血脑屏障模型

Martha A Schalla^1,2,3, Sabrina Oerter^4,5, Alevtina Cubukova⁵

¹Charité Center for Internal Medicine and Dermatology, Department for Psychosomatic Medicine; Charite-Universitätsmedizin BerlinCorporate Member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health, 12203 Berlin, Germany.

|July 29, 2023

概括

调节食欲的类Phoenixin-14不能单独穿越血脑屏障 (BBB). 它的外围影响可能涉及BBB的共同运输机制,需要进一步研究肠-大脑轴.

科学领域:

神经科学是一个神经科学.
内分泌学在内分泌学.
生理学生理学生理学

背景情况:

素-14是一种新发现的食欲调节.
它在胃肠道表达,但它的受体 (GPR173) 位于下丘脑.
外围素-14到达中央受体的机制尚不清楚.

研究的目的:

为了调查素-14是否可以穿越血脑屏障 (BBB).
在体外BBB模型中确定phoenixin-14的透性.

主要方法:

使用了体外单一培养血脑屏障 (BBB) 模型与人类诱导多能干细胞衍生的脑毛细体内皮细胞 (hiPSC-BCECs).
在240分钟内使用ELISA测量素-14通道 (1nM和10nM).
对phoenixin-14.4进行计算的透系数 (PC).

主要成果:

素-14的透系数非常低 (0.021 ± 0.003 微米/分钟为1nM,0.044 ± 0.013 微米/分钟为10nM).
这些值明显低于已知在体内穿过BBB的溶液的值.
素-14 单独不能穿越 BBB.

结论:

素-14不能独立地穿越血脑屏障.
外围素-14的作用很可能依赖于BBB的共同运输机制.
需要进一步的研究,以了解phoenixin-14在肠-大脑轴上的氧化作用.

关键词:

调节食欲调节胃口血脑屏障血脑屏障血脑屏障血脑屏障大脑肠轴脑肠轴胃肠道的胃肠道是什么在下丘脑中,下丘脑在体外 (in vitro) 技术诱导多能干细胞的诱导干细胞.酸是一种酸.菲尼克辛-14的使用方法

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