在dPCD期间的荷尔蒙信号传递:细胞因素作为Solanaceae中基于RNase的自我不相容性的决定因素
Ekaterina Zakharova1, Tatiana Khanina1, Andrey Knyazev1
1All-Russia Research Institute of Agricultural Biotechnology, 127550 Moscow, Russia.
Biomolecules
|July 29, 2023
概括
细胞因素 (CK) 激活花粉管中的类蛋白酶 (CLP),这是植物自我不相容 (SI) 的关键因素. 这种植物激素在Solanaceae植物的SI反应期间调节花粉管生长和编程细胞死亡 (PCD).
科学领域:
- 植物生殖生物学 植物生殖生物学
- 植物自我不相容性的分子机制
- 植物中的植物激素信号传递.
背景情况:
- 自我不相容性 (SI) 是防止开花植物自我受精的关键机制.
- 之前对花的研究已经确定了编程细胞死亡 (PCD) 作为基于S-RNase的SI的因素.
- 假设细胞因素 (CK) 在这个过程中激活卡斯巴类蛋白酶 (CLP).
研究的目的:
- 为了证实该假设,细胞因素 (CK) 激活卡斯巴酶类蛋白酶 (CLPs) 在基于S-RNase的自我不兼容性 (SI).
- 为了研究CK在调节花粉管生长和PCD在SI期间在Solanaceae中的作用.
主要方法:
- 番茄和花斑的外源性泽治疗.
- 在花粉管中对类蛋白酶 (CLP) 活动进行肠道成像.
- 在体外和体内分析雄性性质细胞的发芽和生长.
- 测量细胞质pH值 (PHc) 和F-actin的形成.
- 对IPT5,CKX1和CKX2的基因表达分析.
主要成果:
- 外源性泽治疗激活了花和番茄花粉管中的CLP.
- 克克抑制了雄性性细胞体的发芽和生长,酸化了细胞质pH值,并破坏了细胞骨架.
- 在SI响应过程中,在造型组织中观察到CK积累.
- 在自我不相容的授粉过程中,IPT5基因活性增加,而CKX1和CKX2的表达减少.
结论:
- 细胞因素 (CK) 在SI诱导的PCD机制中起着决定性的作用.
- 基调节花粉管中的关键事件,包括CLP激活,生长抑制和细胞骨变化,有助于SI.
- 这项研究提供了强有力的证据,证明CK在植物SI中作为信号分子的功能.
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