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生物流体微样采集用于治疗药物监测:叙述性审查

Alessia Cafaro¹, Matteo Conti², Federica Pigliasco¹

¹Chromatography and Mass Spectrometry Section, Central Laboratory of Analysis, IRCCS Istituto Giannina Gaslini, 16147 Genoa, Italy.

|July 29, 2023

概括

微量采样为治疗药物监测 (TDM) 和药理动力学研究提供了一个不那么侵入性的替代方案. 创新的设备与敏感的分析技术相结合,可以从小型生物液体体积中准确测量药物度.

科学领域:

实验室医学实验室医学
临床药理学临床药理学
分析化学分析化学

背景情况:

治疗药物监测 (TDM) 优化药物的疗效,并通过保持血度在治疗范围内来减少副作用.
传统的TDM依赖于血或全血样本的静脉穿刺,这可能是侵入性的.
微量采样技术虽然有望减少侵入性采样,但在TDM和药理动力学 (PK) 研究方面面临技术限制.

研究的目的:

批判性地审查各种生物流体的可用微量采样设备.
为在常规临床实践中实施微采样提供信息.
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主要方法:

审查创新的微采样技术,包括3D干燥血液球体,体积吸收微采样 (VAMS),干燥等离子斑点 (DPS) 和微流体装置 (MDS).
评价微采样与高灵敏度分析技术的结合,特别是液态染色学与双重质谱学 (LC-MS/MS) 的结合.
对全血和其他生物液体 (如血,尿液,母乳和唾液) 的微样采样适用性的评估.

主要成果:

创新的微采样设备为传统的采血方法提供了可靠的替代方案.
像LC-MS/MS这样的高灵敏度分析技术可以确保非常小的样本体积的准确性和灵敏度.
微采样,当与先进的分析方法相结合时,解决了以前用于小体积定量化的仪器挑战.

结论:

微量采样为TDM和PK研究提供了一个可行的,不那么侵入性的选择.
集成先进的微采样设备和敏感的分析方法对于成功的临床实施至关重要.
本综述为实验室人员,临床医生和有兴趣采用微采样技术的研究人员提供了有价值的见解.

关键词:

药物监测药物监测是指对药物进行监测.液态色谱学双重质谱学质谱学微量采样微量采样叙事审查叙事审查

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