相关实验视频

Updated: Jun 30, 2026

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

一种两步单复合PCR方法,用于评估患有自闭症谱系障碍的墨西哥年轻人的关键结肠微生物群标记物:协议和试点流行病学应用

Julián Herrera-Mejía¹, Rocío Campos-Vega², Abraham Wall-Medrano¹

¹Instituto de Ciencias Biomédicas, Universidad Autónoma de Ciudad Juárez, Anillo Envolvente del PRONAF y Estocolmo s/n, Ciudad Juárez 32310, Chihuahua, Mexico.

Diagnostics (Basel, Switzerland)

|July 29, 2023

概括

患有自闭症谱系障碍 (ASD) 的儿童表现出明显的肠道微生物群差异. 一种新的两步PCR方法有效地识别了便样本中的关键细菌标记物,将ASD与神经类型个体区分开来.

科学领域:

微生物学微生物学
遗传学是一个遗传学.
神经科学是一个神经科学.

背景情况:

神经系统疾病,包括自闭症谱系障碍 (ASD),与独特的结肠微生物组 (CM) 概况有关.
目前用于分析CM的方法,如16SrRNA测序,是昂贵和耗时的.
识别特定的CM签名可能有助于理解ASD.

研究的目的:

开发和验证一种具有成本效益和快速的方法,用于识别患有自闭症儿童的差异性CM标记.
用一种新的PCR技术,将ASD儿童的CM与神经类型 (NT) 儿童的CM进行比较.

主要方法:

使用标准化的两步PCR协议 (2S-PCR),针对四种候选差异性CM标记物采用了特定的原料:细菌 (BF),菌 (FP),DV和AM.
分析了48名参与者的纯便DNA样本 (39名ASD,9名NT,年龄在3至13岁之间).
用了遗传学分析来证实原料的特异性.

主要成果:

2S-PCR方法成功地在所有便供体中区分了CM生物标志物.
在NT个体中观察到BF,FP和AM的丰度增加.
在ASD患者中观察到DV的丰度增加.

结论:

开发的2S-PCR方法对于识别差异性CM标志物具有敏感性和特异性.
这种方法为ASD研究中CM分析的高通量测序提供了更快,更实惠的替代方案.
独特的CM签名,特别是ASD中DV的丰富性,需要进一步调查.

关键词:

16S rRNA 是一个16S rRNA.在ASD中,使用的是ASD.阿克尔曼西亚的木偶生活.这种细菌是 Bacteroides fragilis.德苏尔福维布里奥尔加里斯 (Desulfovibrio vulgaris) 是一种常见的便细菌 (Faecalibacterium prausnitzii) 是一种有毒的细菌.自闭症自闭症是什么我们的微生物群.两个阶段的PCR.

更多相关视频

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Quantitative Polymerase Chain Reaction-based Analyses of Murine Intestinal Microbiota After Oral Antibiotic Treatment

Published on: November 17, 2018

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

相关概念视频

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

Automated Microbial Diagnostics

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...