反应剂对激活的部分血栓状质蛋白的作用 时间凝块波形分析:多中心研究
Wan Hui Wong1, Chuen Wen Tan1, Nabeelah Binti Abdul Khalid2
1Department of Haematology, Singapore General Hospital, Singapore 169608, Singapore.
Diagnostics (Basel, Switzerland)
|July 29, 2023
概括
基于激活的部分血栓形成时间 (APTT) 的凝块波形分析 (CWA) 显示了试剂特异性的变化. 标准化试剂对于基于APTT的CWA在实验室中的可靠诊断应用至关重要.
科学领域:
- 血液学 血液学 血液学
- 临床化学 临床化学
- 诊断检测试验 诊断检测试验
背景情况:
- 基于激活部分血栓形成时间 (APTT) 的凝块波形分析 (CWA) 提供了超越标准APTT测量的定量见解.
- 预分析变量,特别是试剂效应,尚不清楚,因此需要对CWA的诊断潜力进行标准化.
研究的目的:
- 调查试剂批量变化和实验室间对基于APTT的CWA参数的影响.
- 为基于APTT的CWA建立试剂特定的参考间隔.
主要方法:
- 使用患者样本对三种常见的APTT试剂 (Pathromtin SL,Actin FS,Actin FSL) 进行CWA分析.
- 在Sysmex CS分析器上收集了来自七个亚太地区机构的1055名健康志愿者的CWA数据.
- 使用线性混合模型和非参数方法进行数据分析.
主要成果:
- 在CWA参数中,试剂批量之间的变化不到10%.
- 在同一试剂组内观察到最小的特定部位效应.
- 在不同试剂组中发现了CWA参数的显著差异,需要试剂特定的参考间隔.
- 年龄和性别与CWA参数的相关性较弱.
结论:
- 试剂类型是影响基于APTT的CWA结果的一个重要因素.
- 由于实验室间的差异最小,对相同的凝血计系列进行实验室间数据的聚合是可行的.
- 需要进行进一步的研究,以建立基于APTT的CWA的特定年龄和性别的参考间隔.
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