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从非牛肉食品中分离出来的新型SPHINX/BMMF类DNA序列的识别和描述

Diana Habermann¹, Martin Klempt¹, Charles M A P Franz¹

¹Department of Microbiology and Biotechnology, Max Rubner-Institut, Federal Research Institute for Nutrition and Food, Hermann-Weigmann-Straße 1, 24103 Kiel, Germany.

|July 29, 2023

概括

在各种非牛类食品中发现了新的圆形DNA序列,SPHINX和BMMF. 遗传学分析证实了它们与已知的序列的关系,并揭示了这些群体内不同的亚型.

科学领域:

微生物学微生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

包括SPHINX和BMMF在内的循环复制编码DNA序列是已知的遗传元素.
之前的研究主要在牛来源中对这些序列进行了表征.

研究的目的:

识别和描述非牛类食物来源中的新型循环复制编码DNA序列.
调查这些新型序列的遗传学关系和亚型.

主要方法:

从各种非牛类食品中分离和测序圆形DNA.
用全长基因组序列和Rep蛋白序列进行的家族遗传学分析.
对基因组结构,基因和调控区域 (状重复,dso,sso) 的比较分析.

主要成果:

在猪肉,肉,鱼类,和植物芽中发现了16个新的SPHINX/BMMF相关的圆形DNA序列.
遗传学分析将这些新型序列与已知的SPHINX/BMMF组1和2代表进行了分组.
与Iteron类似的重复分析区分了SPHINX/BMMF组1亚型,而SPHINX/BMMF组2序列形成了两个不同的分类 (A和B).
在SPHINX/BMMF组2序列中发现了与滚动圆复制 (RCR) 元素 (dso,sso) 的同质性,证实了它们的RCR等离子体关系.
对Rep蛋白的分析证实了SPHINX/BMMFRep蛋白与等离子体Rep蛋白的明显关系,2组与RCR等离子体联系在一起,1组可能与甲基复制等离子体联系在一起.

结论:

非牛类食物来源包含与SPHINX/BMMF相关的新型循环DNA序列.
这些发现扩大了SPHINX/BMMF序列的已知宿主范围和多样性.
该研究提供了对这些遗传元素在等离子体生物学更广泛的背景下的进化关系和复制机制的见解.

关键词:

在 BMMFF 里, BMMFF 是一个很好的选择.斯芬克斯 (英语:Sphinx) 是一个神.圆形的 DNA 圆形的 DNA 塑料质粒塑料质粒是什么?滚动圈复制复制滚动圈复制甲基复制是theta的复制.

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