精选可靠的基因参考基因用于基因表达的规范化在三叶草草 (Sagittaria trifolia)
Jing Tang1, Enjiao Li1, Jiexia Liu1
1College of Horticulture and Landscape, Yangzhou University, Yangzhou 225009, China.
Genes
|July 29, 2023
概括
确定稳定的参考基因对于精确的箭头基因表达分析至关重要 (Sagittaria trifolia). 这项研究使用多个算法评估了八个候选基因,为特定组织和发育阶段推UBQ和IDH.
科学领域:
- 分子生物学分子生物学
- 植物科学 植物科学
- 遗传学 是一个遗传学.
背景情况:
- 实时定量PCR (RT-qPCR) 是分析基因表达的一种敏感方法.
- 准确的基因表达分析依赖于选择稳定的参考基因.
- 关于箭头 (Sagittaria trifolia) 适合的参考基因的信息有限.
研究的目的:
- 识别和验证箭头中RT-qPCR最稳定的参考基因.
- 为箭头基因表达研究提供可靠的基因选择参考指导.
主要方法:
- 他们选择了八个候选基因:ACT5,UBQ,GAPDH,CYP,NAC,IDH,SLEEPER和PLA.
- 在各种箭头组织和发育阶段进行RT-qPCR测定.
- 用五种统计算法评估基因表达稳定性:GeNorm,NormFinder,BestKeeper, ΔCt 和 RefFinder.
主要成果:
- UBQ被确定为在多种组织 (叶子,叶茎,根,,) 中的最佳参考基因.
- 在的发育过程中,IDH表达最稳定.
- UBQ和PLA分别是叶子和叶茎发育阶段最稳定的.
结论:
- 建议UBQ和IDH作为可靠的基因参考基因用于箭头中的基因表达研究.
- 这些发现为在箭头研究中选择适当的参考基因提供了关键的指导.
- 经过验证的基因基因可确保组织和发育阶段特定的箭头基因表达分析的准确性.
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