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巨细胞中的营养感知格林受体调节了小鼠中的双A诱导的肠炎症

Xiangcang Ye¹, Zeyu Liu¹, Hye Won Han¹

¹Department of Nutrition, Texas A&M University, College Station, TX 77843, USA.

|July 29, 2023

概括

双甲 (BPA) 暴露通过激活巨细胞促进肠道炎症. 生长激素分泌受体 (GHSR) 对于这种BPA诱导的小鼠炎症反应至关重要.

科学领域:

环境毒理学环境毒理学
免疫学免疫学免疫学
内分泌学在内分泌学.

背景情况:

双,包括双A (BPA),是塑料制造中普遍存在的内分泌干扰环境毒素.
众所周知,BPA会诱发炎症,但其特定的细胞点和机制尚不清楚.
格林通过其受体,生长激素分泌受体 (GHSR) 发出信号,影响新陈代谢和炎症.

研究的目的:

研究BPA对巨细胞的直接影响及其在小鼠肠道内的免疫毒性.
阐明GHSR在BPA介导的炎症反应中的作用.

主要方法:

在暴露于BPA后,在小鼠结肠组织中评估免疫细胞透和促炎基因表达 (Ghsr,Il6,Ccl2).
分析周围血液单核细胞 (PBMC) 和腹膜巨细胞 (PM) 的变化,以检测促炎单细胞和巨细胞的变化.
利用CRISPR基因编辑在RAW264.7巨细胞系中产生GHSR删除突变,以在体外研究BPA的影响.

主要成果:

在小鼠结肠中,BPA暴露增加了免疫细胞透和促炎性基因表达.
短期和长期BPA暴露都增加了循环中的促炎单细胞和巨细胞以及腹腔.
在野生型巨细胞中,BPA促进了亲炎性两极分化,并上调了Ghsr,Il6和Ccl2的表达.
缺乏GHSR的巨细胞对BPA的炎症反应显著减少.

结论:

在巨细胞中,BPA直接促进促炎反应.
GHSR信号传递是BPA诱导的肠道炎症的关键调解者.
针对GHSR信号可能提供一种策略,以减轻BPA的免疫毒性作用,并为生物安全政策提供信息.

关键词:

在 GHSRR 找双 A 是一种双 A.格林激素 (ghrelin) 是一种激素.这是一种炎症炎症炎症炎症.巨细胞是一个巨细胞.

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