对Pb应激反应中的 Salix integra NAC转录因子的全转录组识别和响应模式分析
Yue Xin1, Ruifang Huang2, Meng Xu1
1Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology Ministry of Education, Nanjing Forestry University, Nanjing 210037, China.
International journal of molecular sciences
|July 29, 2023
概括
这项研究在 Salix integra 中确定了 53 个 NAC 转录因子基因,揭示了特定的 NAC 子家族 (ATAF 和 NAP) 在耐受性和植物修复中发挥关键作用. 这些发现为了解植物重金属反应提供了基础.
科学领域:
- 植物分子生物学 植物分子生物学
- 环境科学 环境科学
- 生物技术是生物技术.
背景情况:
- 转录因子家族NAC (NAM-ATAF1/2-CUC) 对植物发育和应激反应至关重要.
- Salix integra 具有重要的 (Pb) 植物修复潜力.
- 了解 Salix integra 中的 NAC 基因功能对于提高其 Pb 耐受性至关重要.
研究的目的:
- 在压下识别和描述 Salix integra 中的 NAC 转录因子转录 (SiNAC).
- 研究SiNACs在 Salix integra对的耐受性中的作用.
- 为未来对反应性SiNAC的功能验证提供基础.
主要方法:
- 使用第三代和下一代的转录基因数据识别了53个SiNAC转录.
- 遗传学分析将SiNAC分为11个子家族.
- 表达式概况分析 (9个时间点) 和短时间序列表达式挖掘器 (STEM) 分析,以评估Pb响应.
- RT-qPCR,细胞下定位实验,以及候选SiNACs的功能预测分析.
主要成果:
- 53个SiNAC中的41个对压力有显著的反应,表现出早期和晚期的积极反应模式.
- ATAF和NAP子家族显示出最显著的反应,特定的SiNAC被确定为候选.
- ATAF子家族的SiNACs准硫和谷的新陈代谢,而NAP子家族的SiNACs准核糖体和烯胺生物合成途径.
- 亚细胞定位证实了精选的SiNAC蛋白质 (SiNAC004,SiNAC120) 的核进口.
结论:
- 两个不同的NAC子家族 (ATAF和NAP) 在 Salix integra.中表现出不同的反应模式.
- 来自ATAF和NAP亚家族的特定SiNAC被确定为耐受性和植物修复的关键参与者.
- 这项研究为 Salix integra 中的耐受性分子机制提供了宝贵的见解,并为基因工程提供了候选者.
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