抗菌耐药性的基因组特征在Staphylococcus pseudintermedius中,从阿根廷和美国的皮肤病犬中分离出来:一项比较研究
Mariela E Srednik1,2, Claudia A Perea1,2, Gabriela I Giacoboni3
1Postdoctoral Research Participation Program, Office of Research in Science and Education, Oak Ridge Associated Universities, Oak Ridge, TN 37831, USA.
International journal of molecular sciences
|July 29, 2023
概括
耐甲素的 Staphylococcus pseudintermedius (MRSP) 是一个越来越令人担忧的狗皮肤炎. 这项研究分析了来自阿根廷和美国的狗的抗菌素耐药性和MRSP的遗传多样性,揭示了多药耐药性和独特的遗传特征.
科学领域:
- 兽医微生物学 兽医微生物学
- 抗微生物耐药性 抗微生物耐药性
- 狗的传染病 狗的传染病
背景情况:
- 伪中间体葡萄球菌 (Staphylococcus pseudintermedius) 是狗群中细菌性皮肤感染的常见原因.
- 耐甲素的 Staphylococcus pseudintermedius (MRSP) 是一种新兴的威胁.
- 了解MRSP的遗传组成和耐药性模式对于有效治疗至关重要.
研究的目的:
- 为了比较抗微生物耐药性 (AMR) 特性和植物遗传关系,从阿根廷和美国的犬类皮肤炎病例中分离出的Staphylococcus pseudintermedius.
- 确定特定的AMR基因,突变和可移动的基因元素,这些基因元素有助于抗药性.
- 分析两个国家之间S. pseudintermedius的种群结构和克隆传播.
主要方法:
- 从阿根廷 (n=29) 和美国 (n=29) 的狗皮肤炎病例中分离和描述S. pseudintermedius.
- 对抗菌素敏感性测试和检测关键的抗菌素耐药性基因 (例如,mecA,blaZ,ermB,tetM).
- 全基因组测序或针对性测序用于遗传学分析,包括SCCmec类型和序列类型 (ST) 确定.
主要成果:
- 62%的分离物体表现出多种药物耐药性.
- 常见的AMR基因包括mecA,blaZ,erMB,dfrG,catA,tetM和aac6' -aph2'.
- 确定了不同的SCCmec类型和序列类型,在两国都确定了ST71,这表明克隆传播.
结论:
- 显著的抗微生物耐药性和多药耐药性在阿根廷和美国的狗皮肤相关的S. pseudintermedius中普遍存在.
- 对比分析显示,在两个地区之间,AMR基因配置文件和SCCmec类型的变化.
- 持续的监测对于监测狗群中耐药性S. pseudintermedius菌株的演变和传播至关重要.
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