抗菌MccM作为Escherichia coliNissle 1917中的主要微素,用于对抗致病性肠道细菌
1School of Biology and Biological Engineering, South China University of Technology, Guangzhou 510006, China.
International journal of molecular sciences
|July 29, 2023
概括
工程化益生菌 Escherichia coli Nissle 1917 (EcN) 过度生产微信M (MccM) 显示了对沙门氏菌的增强抗菌活性. 这种工程 EcN 通过与铁竞争来抑制病原体的生长,提供了对抗肠道感染的潜力.
科学领域:
- 微生物学 微生物学
- 益生菌 益生菌 益生菌
- 细菌因子是一种细菌因子.
背景情况:
- 益生菌 Escherichia coli Nissle 1917 (EcN) 对致病性肠道细菌具有抗菌作用.
- 由EcN产生的微蛋白MccM和MccH47对于它们的抗菌机制至关重要,但人们对其了解甚少.
研究的目的:
- 研究微蛋白MccM和MccH47在EcN抗菌活性中的作用.
- 评估工程 EcN 菌株对抗病原性肠道细菌的潜力.
主要方法:
- 构建和比较EcN突变 (ΔmcmA, ΔmchB, ΔmcmAΔmchB) 与野生型EcN对抗大肠杆菌O157:H7,沙门氏菌和沙门氏菌Typhimurium.
- 在EcN中MmA的过度表达 (EcN::mcmA) 以评估增强的抗菌作用.
- 分析沙门氏菌的粘附和入侵能力,以及巨细胞的炎症反应.
主要成果:
- 对大肠杆菌O157:H7的抗菌作用不受mka或mchB淘汰的影响.
- 对沙门氏菌的抗菌活性在ΔmcmA突变体中显著下降.
- EcN::mcmA证明增强了沙门氏菌生长的抑制,减少了粘附/入侵,并调节了炎症反应.
- 在EcN::mcmA中MccM的过度生产导致铁竞争的 siderophore 产量增加.
结论:
- 微信MccM主要负责EcN对沙门氏菌的活性.
- 工程 EcN 过度生产 MccM 可以被开发为一种益生菌,通过争夺铁来对抗致病性肠细菌的殖民.
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