艾滋病毒和SIV信封糖蛋白与糖脂和脂质相互作用
Rémi Planes1, Elmostafa Bahraoui1
1INFINITY, INSERM, CNRS, CHU Purpan Toulouse, 31024 Toulouse, France.
International journal of molecular sciences
|July 29, 2023
概括
人类免疫缺陷病毒 (HIV) 和猿类免疫缺陷病毒 (SIV) 包裹糖蛋白与多种陶胺结合,包括银糖胺 (GalCer),葡萄糖胺 (GlcCer) 和乳糖胺 (LacCer). 这些特定的相互作用可能会促进病毒进入CD4阴性细胞.
科学领域:
- 病毒学 病毒学
- 生物化学 生物化学
- 细胞生物学 细胞生物学
背景情况:
- 人类免疫缺陷病毒 (HIV) 和猿类免疫缺陷病毒 (SIV) 是导致全球重大健康挑战的晶状病毒.
- 病毒包膜糖蛋白对于介导病毒进入宿主细胞至关重要.
- 控制HIV/SIV附着和进入的精确分子相互作用仍在调查中.
研究的目的:
- 研究HIV-1,HIV-2和SIV包膜糖蛋白与各种陶胺物种的结合相互作用.
- 描述这些相互作用的特异性和生物物理性质.
- 探索这些相互作用在病毒感染性和发病性中的潜在作用.
主要方法:
- 使用放射性标记的病毒葡萄糖蛋白 (I-gp) 和固定或薄层染色学 (TLC) 分离的脂质 (银糖胺[GalCer],葡萄糖胺[GlcCer],乳糖胺[LacCer],胺) 的分子结合试验.
- 通过免疫和非免疫血清和对照蛋白 (I-IgG) 的剂量依赖抑制来评估特异性.
- 物理化学测定涉及糖脂/脂质的单分子薄膜 (朗穆尔薄膜) 作为细胞膜模型,用于测量添加病毒糖蛋白后的表面压力变化.
主要成果:
- 艾滋病毒-1,艾滋病毒-2和SIV包膜糖蛋白与GalCer,GlcCer,LacCer和胺物质相互作用.
- 这些相互作用是特定的,剂量依赖的,可和的,由免疫血清抑制,而不是非免疫血清或无关蛋白质.
- 物理化学测定证实了相互作用,显示病毒糖蛋白添加后脂质膜的表面压力增加.
结论:
- 艾滋病毒和SIV包膜糖蛋白表现出对一系列陶胺的特定结合,包括GalCer,GlcCer,LacCer和陶胺.
- 这些相互作用可以作为替代的附着受体,促进低CD4或CD4阴性细胞的感染.
- 鉴定到的相互作用也可以通过调节宿主细胞信号通路来促进病原性.
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