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纳米LC-EI-MS:生化分析的前景

Natalia Gabrielly Pereira Dos Santos¹, Edvaldo Vasconcelos Soares Maciel¹, Deyber Arley Vargas Medina¹

¹Institute of Chemistry of São Carlos, University of São Paulo, São Carlos 13566-590, Brazil.

International journal of molecular sciences

|July 29, 2023

概括

电子电离质谱法 (EI-MS) 在生物分析中比大气压电离 (API) 具有优势. 新的纳米LC-EI-MS接口使非挥发性分子的分析成为可能,扩大了EI-MS在生物化学中的应用.

科学领域:

分析化学分析化学
生物化学生物化学
质谱测量质量谱测量

背景情况:

液体染色学-质谱学 (LC-MS) 与大气压电离 (API) 是生物分析的标准.
电子电离质谱 (EI-MS) 为识别提供无矩阵效应的可重复光谱.
传统上,EI-MS仅限于挥发性化合物,阻碍其用于重型和非挥发性生物分子.

研究的目的:

审查纳米LC-EI-MS接口技术的进展.
讨论这些新接口的范围和局限性.
介绍纳米LC-EI-MS在生物化学分析中的应用.

主要方法:

关于纳米液体染色学 (nanoLC) 与EI-MS.相结合的最新发展的概述.
对接口策略的讨论:直接EI,液态电子电离 (LEI) 和冷EI.
使用nanoLC-EI-MS.选择的生化分析示例的演示.

主要成果:

纳米LC-EI-MS接口克服了LC和EI-MS之间的兼容性问题.
像直接EI,LEI和冷EI这样的新策略将EI-MS的适用性扩展到非挥发性分子.
在各种生化分析中证明了纳米LC-EI-MS的实用性.

结论:

纳米LC-EI-MS技术为有针对性和无针对性的生化研究提供了显著的分析优势.
这些进步为复杂的生物样本的化合物识别和分析提供了强大的工具.
建立纳米LC-EI-MS有望提高生物化学研究的能力.

关键词:

生物化学分析的方法电子电离化的电离.质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量代谢生物组的代谢生物组纳米液体色谱学纳米液体色谱学

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