增强的细胞壁和细胞膜活动促进了Enterococcus faecium的热适应
Li Wang1,2, Aike Li1, Jun Fang2
1Academy of National Food and Strategic Reserves Administration, Beijing 100037, China.
International journal of molecular sciences
|July 29, 2023
概括
化改善了Enterococcus faecium的生长情况
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
背景情况:
- 菌 (Enterococcus faecium) (E. faecium) 是一种用于物和牲畜料中的益生菌.
- 低耐热性限制了E. faecium在高温食品加工中的应用.
- 菌株化为开发耐热菌株提供了一种具有成本效益的方法.
研究的目的:
- 通过化开发一种耐高温的E. faecium菌株.
- 调查增强耐热性的潜在机制.
主要方法:
- 从45°C到70°C的E. faecium逐渐进行热处理.
- 野生型 (WT) 和养型 (RS047-wl) 菌株的比较分析.
- 转录组,代谢组和蛋白组分析.
主要成果:
- 与WT RS047相比,化的RS047-wl菌株在65°C下40分钟的生存率是WT RS047的11.5倍.
- RS047-wl表现出其细胞膜中的和脂肪酸含量增加和更大的细胞体积.
- 多omics分析揭示了RS047-wl. 的增强细胞壁和膜合成.
结论:
- 增强的细胞壁和膜合成是RS047-wl.的高温耐受性的关键因素.
- 改进的合成在热应激下保持正常的细胞形态.
- 这项研究为在食品工业中应用耐热E. faecium提供了基础.
更多相关视频
06:36Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds
Published on: September 8, 2021
2.8K
07:23Measuring the Effects of Bacteria and Chemicals on the Intestinal Permeability of Caenorhabditis elegans
Published on: December 3, 2019
14.6K
相关概念视频
Factors Influencing Microbial Growth: Temperature
68
Microorganisms display remarkable adaptations, enabling them to thrive in diverse ecological niches across a wide range of temperatures. Temperature profoundly influences microbial growth by affecting enzymatic activity, membrane fluidity, and other cellular processes.Each microorganism operates within a specific temperature range defined by three cardinal points: minimum, optimum, and maximum. Below the minimum temperature, membranes lose fluidity, halting transport processes. Above the...
68
Diversity of Archaea IV
51
Hyperthermophilic archaea are a group of extremophiles thriving at temperatures above 80°C, often in hydrothermal vents and volcanic soils where conditions surpass the boiling point of water. At such temperatures, proteins, membranes, and DNA in most organisms degrade, but hyperthermophiles have evolved remarkable adaptations to maintain stability and function.Unique Cellular FeaturesHyperthermophilic membranes are composed of a monolayer of biphytanyl tetraether lipids, which resist...
51
Diversity of Archaea III
32
Crenarchaeota, a prominent phylum of Archaea, is remarkable for its ability to thrive in extreme environments characterized by high temperatures and acidity. These microorganisms inhabit sulfuric hot springs, volcanic systems, and submarine hydrothermal vents, where temperatures often exceed 100°C. The unique adaptations of Crenarchaeota not only allow survival under such extreme conditions but also provide insights into the mechanisms of life in primordial Earth-like...
32
Archaeal Cell Wall
49
Archaeal cell walls are structurally and compositionally distinct from their bacterial counterparts, lacking the characteristic peptidoglycan layer found in most bacteria. Instead, archaeal cell walls exhibit remarkable diversity, utilizing materials such as pseudomurein, polysaccharides, and proteins to construct their protective outer layers. This structural flexibility is closely tied to archaea's ecological adaptability.S-Layers: The Common Archaeal Cell WallThe S-layer is the most...
49
Bacterial Cell Wall
59
The bacterial cell wall is an essential structural component that encases the plasma membrane, preserving cellular integrity, determining shape, and protecting against osmotic stress. This rigid yet flexible structure primarily comprises peptidoglycan, a polymer that forms a mesh-like matrix conferring mechanical strength and flexibility.Peptidoglycan Composition and StructurePeptidoglycan, the core of the bacterial cell wall, comprises alternating units of N-acetylglucosamine (NAG) and...
59
Transduction
42
Among the three main modes of HGT—transformation, conjugation, and transduction—transduction is unique in that it is mediated by bacteriophages, or bacterial viruses.Transduction occurs in two ways. Generalized transduction occurs during the lytic cycle of a bacteriophage infection. In this process, bacteriophages infect bacterial cells, replicate within them, and ultimately cause cell lysis, releasing newly assembled virions. Occasionally, random fragments of the bacterial genome...
42
