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AFB₁ 微生物降解由Bacillus subtilis WJ6及其降解机制基于比较转录学方法的探索

Peizhou Yang¹, Wenjing Wu¹, Danfeng Zhang¹

¹Anhui Key Laboratory of Intensive Processing of Agricultural Products, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230601, China.

|July 29, 2023

概括

细菌细菌WJ6有效地通过分泌的酶降解了阿弗拉托xin B1. 这种生物排毒方法提供了一种安全而有效的解决方案,以防止非洲毒素污染,保护人类和动物的健康.

科学领域:

微生物学微生物学
生物技术是生物技术.
环境科学环境科学

背景情况:

非洲毒素污染对人类和动物健康构成重大风险,导致巨大的经济损失.
使用微生物和酶的生物排毒提供了一种特定,高效和环保的方法来缓解亚毒素污染.

研究的目的:

选和识别一种具有高毒素B1降解能力的微生物菌株.
为了研究由已识别的菌株对阿弗拉托克辛降解的潜在分子机制.

主要方法:

使用分子,生理和生化技术查和识别Bacillus subtilis WJ6.
通过发酵,无细胞超浮剂和细胞悬浮剂评估阿弗拉托克辛B1降解效率.
进行比较性转录组学分析,以识别对亚拉托克素B1暴露的反应中差异表达的基因.

主要成果:

细菌细菌WJ6在降解阿弗拉托克辛B1方面表现出高效率,降解率为81.57% (发酵),73.27% (无细胞超浮物) 和8.39% (细胞悬浮物).
转录基因分析显示,B. subtilis WJ6在暴露于亚毒素B1.1后,有60个上调调节的和31个下调调节的基因.
基因实体学分析表明,上调的基因与细胞聚合和结构分子活动有关,而下调的基因与多生物体过程有关.

结论:

细菌细菌WJ6有效降解亚拉托克辛B1主要通过细胞外酶的分泌.
降解过程涉及基因表达的显著变化,包括与结构分子活动相关的基因的上调和参与多个生物体过程的基因的下调.

关键词:

这种细菌是 Bacillus subtilis.非洲毒素B1是什么?生物解毒生物解毒.比较的转录组学进行比较微生物降解微生物降解

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