内源性CO2过压对高酒精代谢的影响在泡葡萄酒生产过程中
María Del Carmen González-Jiménez1, Juan Carlos Mauricio1, Jaime Moreno-García1
1Department of Agricultural Chemistry, Edaphology and Microbiology, Agrifood Campus of International Excellence CeiA3, University of Cordoba, Ctra. N-IV-A, km 396, 14014 Cordoba, Spain.
二氧化碳过压会影响泡葡萄酒发酵过程中的Saccharomyces cerevisiae代谢. 这项研究揭示了不同的蛋白质组和代谢组概况,影响了更高的酒精产量和酵母行为.
科学领域:
- 葡萄酒学 葡萄酒学 葡萄酒学
- 微生物的代谢物.
- 蛋白质组学是指蛋白质组学
背景情况:
- 较高的酒精含量显著影响起泡葡萄酒的香味和味道.
- 在发酵过程中二氧化碳 (CO2) 过度压力对Saccharomyces cerevisiae的代谢影响尚未完全理解.
研究的目的:
- 在内源性CO2过压下研究Saccharomyces cerevisiae中的蛋白质和代谢变化.
- 了解二氧化碳过压,较高的酒精形成和起泡葡萄酒生产过程中的酵母代谢之间的关系.
主要方法:
- 使用蛋白质组学和代谢组学方法.
- 采用OFFGEL分离和LTQ Orbitrap用于蛋白质识别.
- 使用GC-FID和SBSE-TD-GC-MS量化较高的酒精,并通过HPLC通过氨基酸.
主要成果:
- 在二氧化碳过压和控制条件下检测到甲醇和六种更高的酒精.
- 在CO2过压下确定了15种蛋白质,在控制条件下确定了22种蛋白质.
- 在这两种条件之间观察到不同的代谢和蛋白质基因概况,表明CO2过压会影响更高的酒精代谢.
结论:
- 在酒发酵过程中,二氧化碳过压会影响Saccharomyces cerevisiae的代谢和蛋白质组概况.
- 前体和高酒精之间的直接相关性只有在没有CO2过压的情况下才被确立.
- 这些发现为了解起泡葡萄酒的第二次发酵中的酵母代谢-蛋白质动态提供了基础.
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