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受到水力压裂和环境因素影响的微生物群落在现场煤炭储内

Yang Li^1,2,3, Jian Chen^1,2, Shuheng Tang^4,5,6

¹School of Earth and Environment, Anhui University of Science and Technology, Huainan 232001, China.

|July 29, 2023

概括

水力压裂增强了煤矿库中的微生物群落,促进了有机降解和碳循环,从而增加了煤床甲的产量. 这一过程改善了微生物的条件,有助于甲和二氧化碳的循环.

科学领域:

*地质微生物学和水库工程
* 地下环境的微生物生态学

背景情况:

* 煤床甲 (CBM) 生物工程利用微生物的作用在碳循环中转化和利用二氧化碳.
* 地下煤炭储水库是复杂微生物群落的宿主,这些微生物群落是由它们的环境塑造的.
* 对水库微生物的实验室研究不能完全复制现场条件和相互作用.

研究的目的:

* 分析水力压裂后煤矿库中的微生物反应.
* 了解水力压裂如何影响微生物社区的结构和在现场的功能.
* 调查水力压裂在增强微生物增强煤床甲 (MECBM) 生产中的作用.

主要方法:

* 16S rRNA测序技术的应用.
*分析了盆地水力压裂井的煤床甲生产水样.
* 微生物群落在破裂井和非破裂井中的比较.

主要成果:

*水力压裂显著改善了参与有机降解的微生物群落的多样性.
* 在断裂后观察到,Actinobacteria,Aminicenantes和Planctomycetes的丰度显著增加.
* 甲素的多样性和丰富性,特别是在破裂井中,受到生态因素的影响,影响代谢途径.
* 观察到水力压裂对微生物群落的影响持续数月.

结论:

*水力压裂对煤矿库中的微生物群落产生积极影响,增强有机降解和碳循环.
*观察到的微生物变化加强了甲和二氧化碳的循环,支持MECBM.
* 这项研究为优化微生物增强的煤床甲回收通过液压压裂提供了理论基础.

关键词:

生物地质化学生物地质化学煤床甲的使用情况甲氧化物是一种甲基化物.微生物多样性的微生物多样性这是水盆地.

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