普西迪亚瓜亚瓦水性提取物对敏感和耐药细菌菌株的抗菌活性
Geraldo Augusto Pereira1, Douglas Siqueira de Almeida Chaves2, Taynara Monsores E Silva1
1Pos Graduation Program of Veterinary Science, Veterinary Institute, Federal Rural University of Rio de Janeiro, BR 465, km 7, Seropédica 23897-000, RJ, Brazil.
Microorganisms
|July 29, 2023
概括
果叶提取物显示抗菌活性对抗耐药性格拉姆阳性细菌. 这种植物药物为应对新出现的微生物耐药性提供了一个有希望的替代方案.
科学领域:
- 药物鉴定学和微生物学
- 自然产品化学 自然产品化学
- 抗微生物耐药性研究研究
背景情况:
- 抗微生物药物耐药性是一个日益严重的全球健康问题,由包括不适当的抗微生物药物使用在内的因素驱动.
- 植物药物和草药,如那些来自*Psidium guajava* (瓜) 的药物,为对抗耐药微生物提供了潜在的替代品.
- 以前的研究表明瓜提取物具有抗菌潜力,但对抗耐药菌株的活性数据有限.
研究的目的:
- 为了评估Psidium guajava叶的水性提取物对野生型和耐药细菌菌株的抗菌能力.
- 描述果叶提取物的化学成分,包括和素含量,以及抗氧化活性.
主要方法:
- 高性能液体染色学 (HPLC) 用于确定总,素和化学成分.
- 使用EC50值来评估抗氧化能力.
- 通过磁盘扩散和微稀释方法评估抗菌活性,以对抗阳性 (*金黄色葡萄球菌*, *伪中间葡萄球菌*, *杆菌* 种类) 进行评估. 和格拉姆阴性 (*Escherichia coli*) 细菌,包括耐药菌株.
主要成果:
- 水性瓜叶提取物含有17.02 ± 6.87 mg/g 干燥提取物总和14.09 ± 1.20 mg 酸等价物/g 干燥提取物总.
- 观察到中等的抗氧化能力,EC50为140μg/mL. 黄类化合物 (鲁丁,hesperidin, quercetin) 是主要的化合物.
- 提取物对敏感和耐药的格拉姆阳性细菌表现出显著的抗菌活性,最小抑制度 (MIC) 为6.8 mg/mL. 没有观察到对大肠杆菌的活性.
结论:
- *Psidium guajava* 叶子的水性提取物具有显著的抗菌活性,可以对敏感和耐药的阳性细菌产生抗菌作用.
- 观察到的增强活性可能归因于提取物中高度的总和素.
- 这些发现突显了瓜亚娃叶提取物作为天然治疗资源的潜力,用于对抗抗性微生物菌株.
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