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通过基于NMR的代谢学研究希腊格拉维埃拉奶酪

Evangelia Ralli¹, Apostolos Spyros¹

¹NMR Laboratory, Department of Chemistry, University of Crete, Voutes Campus, 710 03 Heraklion, Crete, Greece.

Molecules (Basel, Switzerland)

|July 29, 2023

概括

这项研究使用了1H NMR光谱和化学测量来分析格拉维埃拉奶酪代谢物. 像GABA和脂肪酸这样的特定化合物根据原产地区分奶酪,帮助认证PDO真实性.

科学领域:

食品化学食品化学
分析化学分析化学
代谢学代谢学代谢学

背景情况:

格拉维埃拉奶酪是一种流行的希腊硬奶酪,具有独特的特点,受到地理,牛奶类型和生产方法的影响.
保护原产地名称 (PDO) 地位强调了地理原产地和传统做法在奶酪生产中的重要性.

研究的目的:

用1HNMR光谱和化学测量来确定来自不同地理位置的格拉维埃拉奶酪的代谢物概况.
根据其原产地,特别是克里特岛内,确定区分格雷维埃拉奶酪的关键化合物.
探索化学模型的潜力,以验证PDO葡萄酒产品的真实性.

主要方法:

核磁共振 (NMR) 光谱法用于分析格拉维埃拉奶酪的极和脂质分数.
使用化学测量技术,包括差异分析 (Anova),有针对性的差异分析和正交部分最小平方差异分析 (OPLS-DA).
进行了有机酸,氨基酸和脂肪酸 (FA) 的量化.

主要成果:

来自不同地区的格拉维埃拉奶酪的代谢物概况存在显著差异.
确定了γ-氨基黄油酸 (GABA),合烯酸 (CLA) 和烯酸是克里特 gravieras 的关键区分因素.
来自克里特东部的格拉维埃拉奶酪的总氨基酸含量更高.
区分分析模型成功地根据脂质和氨基酸配置文件对来自希腊大陆,冰岛岛和克里特岛的 gravieras 进行了分类.
OPLS-DA模型证明了在克里特岛内区分 gravieras 的能力.

结论:

使用NMR光谱和化学测量的代谢物分析是有效的在区分基于地理来源的格拉维埃拉奶酪.
特定的代谢物,如GABA和某些脂肪酸可以作为地理认证的标记.
开发的化学测量模型显示,在验证原产品的真实性方面,有很大的潜力.

关键词:

核磁共振光谱法 (NMR) 是一种光谱法.氨基酸是氨基酸中的一种.脂肪酸脂肪酸脂肪酸脂肪酸格拉维埃拉奶酪是一种格拉维埃拉奶酪.代谢物形状的代谢物形状代谢生物组的代谢生物组多变量分析多变量分析.

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