菲塞丁通过通过Sirt1路径调节氧化应激和线粒体功能来延迟排卵后卵细胞衰老
Xupeng Xing1,2,3, Yalin Liang1,2,3, Yanan Li1,2,3
1National Engineering Research Center for Breeding Swine Industry, South China Agricultural University, Guangzhou 510642, China.
Molecules (Basel, Switzerland)
|July 29, 2023
概括
菲塞丁是一种天然化合物,通过增加Sirt1水平,延迟了小鼠的卵老化. 这改善了卵子的发育,并减少了氧化应激,为辅助生殖提供了潜在的好处.
科学领域:
- 生殖生物学 生殖生物学
- 分子生物学分子生物学
- 老年学是一门学科.
背景情况:
- 卵细胞质量对于胚胎发育和受精成功至关重要.
- 排卵后卵细胞衰老会对受精率和胚胎发育产生负面影响,特别是在辅助生殖技术中.
- 确定缓解卵细胞衰老的干预措施对于改善生殖结果至关重要.
研究的目的:
- 在小鼠模型中研究素的潜力,一种饮食中的黄醇,在延迟排卵后卵细胞衰老方面.
- 阐明菲塞对卵细胞衰老的影响的分子机制,重点关注Sirt1和氧化应激通路.
主要方法:
- 用菲塞治疗老老鼠卵细胞.
- 评估受精率,胚胎发育和卵细胞质量标志物.
- 分析Sirt1表达,谷氨 (GSH) 水平,活性氧物种 (ROS) 积累和线粒体功能.
- 评估分子变化,包括螺旋体形态,亡,H3K9me3表达和线粒体基因表达 (Tfam,Co2,Atp8).
主要成果:
- 菲塞显著延迟了小鼠的卵细胞的卵巢过期衰老.
- 菲塞丁治疗改善了受精后老化的卵细胞的发育潜力.
- 菲塞丁上调了Sirt1,增加了GSH水平,并减少了ROS积累,从而抑制了氧化应激.
- 菲塞丁减轻了因衰老引起的螺旋异常,线粒体功能障碍和亡,同时降低了异常的H3K9me3表达.
- 菲塞通过上调Sirt1来增强线粒体基因表达,这被Sirt1抑制的影响逆转所证实.
结论:
- 菲塞有效地延迟了小鼠的卵子细胞的排卵后衰老.
- 菲塞的抗衰老作用是通过Sirt1的上调调节来调节的,从而减少氧化应激和改善卵细胞质量.
- 菲塞丁代表了一种有前途的治疗剂,可以缓解与年龄相关的卵细胞质量下降,并在辅助生殖环境中增强生殖成功.
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