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在Staphylococcus aureus菌株中检测到普特雷素

Javier Seravalli¹, Frank Portugal²

¹Redox Biology Center and Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, USA.

Pathogens (Basel, Switzerland)

|July 29, 2023

概括

黄金葡萄球菌 (黄金葡萄球菌) (S. aureus) 可以产生聚胺,这是生命必不可少的聚胺,这与之前的假设相反. 这项研究证实了S. aureus在各种菌株中合成瑞素和N-乙-瑞素.

科学领域:

微生物学微生物学
生物化学生物化学
分子生物学分子生物学

背景情况:

聚氨酸,如氨酸,对于古生物,细菌和真核生物的细胞功能至关重要.
以前人们认为,包括黄金葡萄球菌 (S. aureus) 在内的阳性细菌缺乏素合成途径.
这挑战了对细菌中聚胺生物合成的既定理解.

研究的目的:

调查黄金葡萄球菌菌株合成普特雷辛及其衍生物的能力.
为了确定从脑中临床分离的金黄色细菌是否会产生putrescine.
为了阐明涉及到S. aureus.中putrescine生产的代谢途径.

主要方法:

在化学定义介质 (CDM) 中培养多个S. aureus菌株 (ATCC,NCBI,临床分离物).
使用质谱法 (MS) 分析条件介质 (CM) 来量化聚胺含量.
使用13C/15N标记的氨酸作为代谢追踪剂,确认了氨酸的合成.

主要成果:

所有评估的黄金菌株,包括临床分离物,都显示出能够产生普特雷斯和/或N-乙-普特雷斯.
代谢追踪证实了S. aureus.中从氨酸中新生合成布特雷辛的新生合成.
标记的代谢物包括阿格马丁,N-乙-普特雷斯,奥尼丁,素,プロ林和氨 (NH3).
没有任何菌株产生多氨酸精氨酸或精氨酸.

结论:

黄金葡萄球菌具有合成普特雷斯及其衍生物N-乙-普特雷斯的能力.
这一发现纠正了之前的假设,即S. aureus缺乏putrescine生物合成.
这项研究突出了黄金菌的代谢灵活性及其在感染期间在聚胺代谢中的潜在作用.

关键词:

这是一种N-乙-丁素.黄金葡萄球菌黄金葡萄球菌细菌病原体是一种细菌病原体.质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量多重反应监测 (MRM) 是指多重反应的监测.布特雷斯基因 (Putrescine) 是一种有毒的药物.

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