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来自人类肝脏的囊:分子特征和功能分析

Amornrat Geadkaew-Krenc¹, Rudi Grams¹, Sinee Siricoon²

¹Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Khlong Luang, Pathum Thani 12120, Thailand.

Pathogens (Basel, Switzerland)

|July 29, 2023

概括

奥皮斯托吉斯维弗里尼的cystatins OvCys1,3,和4 抑制宿主cathepsins,但不是抗原的. 这些发现表明,寄生虫囊素可能不是对抗肝炎感染的免疫反应的关键标.

科学领域:

寄生虫学的寄生虫学
分子生物学分子生物学
生物化学生物化学

背景情况:

人类肝脏,Opisthorchis viverrini,与泰国的胆管癌有关.
寄生虫分泌物,特别是蛋白酶,与疾病发展有关.
囊蛋白酶抑制剂 (囊蛋白质) 是蛋白酶活性的关键调节剂.

研究的目的:

为了在分子层面上描述Opisthorchis viverrini的特征,我们需要:cystatins.
为了研究选择的cystatins的抑制活性和抗原性质.

主要方法:

使用RT-PCR克隆了六个Opisthorchis viverrini cystatin (OvCys1-6) 的cDNA序列.
西部斑点分析检测虫提取物和分泌分泌 (ES) 产品中的囊素.
酶抑制测定和间接ELISA来评估免疫反应.

主要成果:

鉴定了六种OvCystatincDNA,分为1型 (OvCys1-2) 和2型 (OvCys3-6).
在寄生虫提取物和ES产品中检测到OvCys1,OvCys3和OvCys4.
这三种囊素在广泛的pH范围内有效抑制哺乳动物甲素L,并且具有热稳定性.
不同于Fasciola gigantica的cystatins,没有观察到受感染的仓鼠血清的免疫反应.

结论:

奥皮斯托希斯·维弗里尼的cystatinsOvCys1,3,和4表现出强大的囊蛋白酶抑制活性.
这些囊素不是寄生虫ES产品中的主要抗原成分.
需要进一步的研究来探索Opisthorchis viverrini的cystatins在寄生虫病原性中的其他潜在作用.

关键词:

这就是Opisthorchis viverrini.在 cathepsin 中.囊类药物囊类药物囊蛋白酶抑制剂囊蛋白酶抑制剂免疫反应性免疫反应性抑制抑制抑制的抑制作用

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