在寒冷压力期间对Campylobacter jejuni和Campylobacter coli进行转录组分析
Anand B Karki1, Bhuwan Khatri2, Mohamed K Fakhr1
1Department of Biological Science, The University of Tulsa, Tulsa, OK 74104, USA.
Pathogens (Basel, Switzerland)
|July 29, 2023
概括
低温对Campylobacter基因表达的影响在物种之间不同. 大肠杆菌聚焦于鞭毛组装,而杆菌修改了核糖体功能,这给食品安全带来了挑战.
科学领域:
- 食品微生物学 食品微生物学
- 细菌基因组学是一种细菌基因组学.
- 食品安全科学 食品安全科学
背景情况:
- 坎比洛巴克特菌种导致坎比洛巴克特菌病,这是一个严重的公共卫生问题.
- 在冷却温度 (4°C) 上,坎皮洛巴克特菌的长期存活带来了食品安全方面的挑战.
- 坎皮洛巴克特经常出现在零售肉类和肝脏产品中.
研究的目的:
- 在低温 (4°C) 下研究大肠杆菌和杆菌的转录基因反应.
- 为了比较基因表达模式在不同的媒体 (汁和穆勒-欣顿) 和随着时间的推移.
- 为了确定关键的基因和受冷影响的通路在食物传播病原体.
主要方法:
- 使用RNA测序 (RNA-seq) 来分析大肠杆菌HC2-48 (Cc48) 和大肠杆菌OD2-67 (Cj67) 的转录组.
- 在0,0.5,24和48小时在4°C化时采集样本.
- 基因表达在两个不同的介质中进行了分析:富含营养的汁 (CJ) 和穆勒-亨顿 (MHB).
主要成果:
- 低温在大肠杆菌中显著影响了鞭毛组装基因,而在C. jejuni.中则调节了核糖体和核糖核蛋白复合体基因.
- 大多数在4°C处差异表达的基因 (DEGs) 在不同化时间内都没有一致表达.
- 与MHB相比,在CJ中观察到更多的DEG,但在所有时间点都没有表达任何共同的基因.
结论:
- 寒冷的温度会在C. coli和C. jejuni.之间明显地影响Campylobacter基因表达.
- 食物矩阵 (CJ) 比实验室介质 (MHB) 更多地影响差异性基因表达,但不是唯一的因素.
- 了解这些寒冷适应机制对于制定有效的食品安全策略来对抗Campylobacter至关重要.
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