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作为新的抗菌剂的烯和烯基表面活性剂:特征,自我聚合特性,以及DPPC/表面活性剂囊泡配方
Zakaria Hafidi1,2, Lourdes Pérez1, Mohammed El Achouri2,3
1Department of Surfactants and Nanobiotechnology, IQAC-CSIC, 08034 Barcelona, Spain.
Pharmaceutics
|July 29, 2023
概括
从氨酸和氨酸合成的阴阳性表面活性剂显示出有前途的抗菌活性. 它们被纳入脂质二层会影响有效性,有些配方保留强大的抗菌特性.
科学领域:
- 绿色化学和材料科学 绿色化学和材料科学
- 生物物理化学 生物物理化学
- 抗微生物研究的研究.
背景情况:
- 来自氨基酸的阴离子表面活性剂提供了可持续的替代品.
- 了解它们的聚合和生物活性对于应用至关重要.
- 基于氨基酸的表面活性剂可以表现出独特的物理化学和生物特性.
研究的目的:
- 从氨酸和氨酸合成和表征新型阴离子表面活性剂.
- 为了研究它们的酸性质,聚合行为和抗菌功效.
- 探索它们与脂质双层的相互作用和潜在的作用机制.
主要方法:
- 氨酸 (CnPC3NH3Cl) 和氨酸 (CnTC3NH3Cl) 表面活性剂的绿色合成.
- 导电性和光度的测量,以确定关键的微粒度 (CMCs).
- 显而易见的pKa确定的电位计定位.
- 对八种细菌菌株进行抗菌检测.
- 分子对抗酸甘油糖酶转移酶 (PDB ID:2OQO) 的对接.
- 微角X射线散射 (SAXS) 和密度函数理论 (DFT) 用于研究表面活性剂-活体相互作用.
主要成果:
- 表面活性剂通过绿色途径成功合成.
- 对于CnPC3NH3Cl而言,CMC范围在0.42-16.2毫米之间,对于CnTC3NH3Cl而言,CMC范围在0.29-4.6毫米之间.
- 酸度随着链条长度的增加而增加,对于氨衍生物的酸度略高.
- 观察到有前途的广泛抗菌活性.
- 分子对接表明了与丁糖甘糖酶转移酶的潜在结合相互作用.
- 萨克斯表示将其纳入DPPC双层,而不会造成重大破坏.
- 特定的C12TC3NH3Cl/DPPC配方表现出良好的活性,而其他配方则失去了有效性,可能是由于强烈的表面活性剂-bilayer亲和力 (DFT).
结论:
- 基于氨酸和氨酸的阴离子表面活性剂是有效的抗菌剂.
- 它们的抗菌活性可以通过配制脂质双层来调节.
- 这些表面活性剂与DPPC双层之间的强烈亲和力可能会降低它们在某些配方中的有效性.
- 这些化合物代表了开发新抗菌剂的有希望的候选者.
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