双体系统中黄素和α-托科菲罗尔的联合封装:物理化学性质和生物活性
Daniela Vergara1, Olga López2, Claudia Sanhueza1
1Center of Excellence in Translational Medicine-Scientific Technological Bioresource Nucleus (CEMT-BIOREN), Faculty of Medicine, Universidad de La Frontera, Casilla 54-D, Temuco 4780000, Chile.
Pharmaceutics
|July 29, 2023
概括
一种新的双体体系统有效地联合封装黄素和α-托科菲罗尔,以改善局部输送. 这种创新的载体表现出显著的抗氧化和抗微生物特性,显示出皮肤组织治疗的前景.
科学领域:
- 纳米技术 纳米技术
- 材料科学 材料科学 材料科学
- 皮肤病学 皮肤病学
背景情况:
- 由于可溶性和稳定性差,黄素 (cur) 和α-托科菲罗尔 (α-toc) 的局部应用面临限制.
- 开发先进的输送系统对于提高这些抗氧化化合物的疗效至关重要.
- 双细胞体,一种新的系统,将双细胞体结合在脂质体内,为联合封装提供了一个潜在的解决方案.
研究的目的:
- 开发和描述一种新的双体体系统,用于黄素和α-托科菲罗尔的联合封装.
- 评估cur/α-toc-bicosomes的物理化学特性,抗氧化活性和生物兼容性.
- 评估双体在局部输送和皮肤疾病治疗方面的潜力.
主要方法:
- 使用DPPC,DHPC,cur和α-toc.制备含有黄素和α-托科菲罗尔的双.
- 使用Lipoid P-100和胆固醇形成了封装这些双细胞的脂质体囊泡.
- 评估了物理化学特性 (大小,EE,LC),抗氧化活性,细胞活力和对C. albicans的抗菌作用.
主要成果:
- 黄素和α-托科菲罗尔在双细胞 (<20 nm) 中成功溶解,形成二元体 (303-420 nm).
- 实现了高封装效率 (56-77%的cur,51-65%的α-toc) 和负载能力 (52-67%).
- 比科索姆显著降低了脂质氧化 (52%),增强了抗氧化活性 (60%),表现出良好的细胞活力 (>85%在纤维细胞中,≥65%在角质细胞中),并抑制了C. albicans的生长 (33-76%).
结论:
- 双体系统代表了一种新且有效的携带器,用于共同封装,溶解,保护和传递黄素和α-托科菲罗尔等抗氧化分子.
- 协同的抗氧化和抗微生物作用,加上生物相容性,突显了cur/α-toc-bicosomes在治疗与氧化压力相关的皮肤问题和C. albicans感染方面的潜力.
- 进一步的体外和体内研究是有必要的,以充分确定这些bicosome配方的皮肤应用的疗效和安全性.
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