一项全基因组关联研究揭示了牛中与种子蛋白质含量相关的区域
Yilin Chen1, Haizheng Xiong1, Waltram Ravelombola2
1Department of Horticulture, University of Arkansas, Fayetteville, AR 72701, USA.
Plants (Basel, Switzerland)
|July 29, 2023
概括
研究人员使用全基因组关联研究 (GWAS) 确定了七个与牛种子蛋白质含量相关的显著单核酸多态 (SNP) 标志物. 这些发现可以加速牛种植,以改善营养和产量.
科学领域:
- 农业科学 农业科学
- 遗传学 遗传学 是一个
- 植物育种 植物育种
背景情况:
- 牛 (Vigna unguiculata) 是发展中国家的重要蛋白质来源,需要进行基因改进以提高营养价值.
- 识别控制种子蛋白质含量的遗传位置对于高效的牛育种计划至关重要.
研究的目的:
- 进行全基因组关联研究 (GWAS),以确定与牛种子蛋白质含量相关的遗传标记物.
- 执行基因组预测 (GP) 来评估预测种子蛋白质含量用于育种应用的准确性.
主要方法:
- 在使用110,155个单核酸多态 (SNP) 的161个牛加入上进行了GWAS.
- 用五种GWAS模型 (SMR,GLM,MLM,FarmCPU,BLINK) 来识别重要的标记.
- 用五种模型 (rrBLUP,BRR,BA,BB,BL) 进行基因组预测,具有各种标记密度.
主要成果:
- 确定了七个与种子蛋白质含量相关的显著SNP标记物,所有这些都位于8号染色体上.
- 鉴定到的基因位与硫素超级家族基因Vigun08g039200有关,该基因对蛋白质含量和质量至关重要.
- 在不同的模型和标记集中,基因组预测准确度从42.9%到52.1%不等.
结论:
- 已识别的SNP标记物和相关基因为牛繁殖中标记辅助选择提供了有价值的标.
- 基因组预测可以通过启用早期性能预测来加速繁殖周期,有助于开发高蛋白牛品种.
- 这些发现对改善牛的营养质量和支持粮食安全具有重大意义.
更多相关视频
10:24Author Spotlight: Quantification of Aflatoxins and Phytoalexins in Peanut Seeds to Identify Genetic Resistance Against Aspergillus
Published on: April 19, 2024
1.2K
07:19Quantifying Plant Soluble Protein and Digestible Carbohydrate Content, Using Corn Zea mays As an Exemplar
Published on: August 6, 2018
19.9K
相关概念视频
Genome-wide Association Studies-GWAS
13.6K
Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...
GWAS does not require the identification of the target gene involved in...
13.6K
Single Nucleotide Polymorphisms-SNPs
15.3K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
15.3K
Western Blotting
16.5K
Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
16.5K
Seed Structure and Early Development of the Sporophyte
28.4K
Seed structures are composed of a protective seed coat surrounding a plant embryo, and a food store for the developing embryo. The embryo contains the precursor tissues for leaves, stem, and roots. The endosperm and cotyledons—seed leaves—act as the food reserves for the growing embryo.
28.4K
Plant Breeding and Biotechnology
19.0K
Crop cultivation has a long history in human civilization, with records showing the cultivation of cereal plants beginning at around 8000 BC. This early plant breeding was developed primarily to provide a steady supply of food.
19.0K
