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在Aegilops_tauschii中对m6A写字,阅读和删除器的全基因组识别,表达和进化分析

Huiyuan Lin¹, Tingrui Shi¹, Ying Zhang¹

¹State Key Laboratory of Crop Stress Biology in Arid Areas, College of Agronomy and Yangling Branch of China Wheat Improvement Center, Northwest A&F University, Yangling 712100, China.

Plants (Basel, Switzerland)

|July 29, 2023

概括

这项研究确定了Aegilops tauschii中25个N6-甲基氨酸 (m6A) 基因,揭示了它们在植物生长和压力中的作用. 遗传分析显示,在小麦化过程中m6A基因变异,为作物改进提供了洞察力.

科学领域:

植物分子生物学植物分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
基因组学就是基因组学.

背景情况:

N6-甲基氨酸 (m6A) 修改对植物生长,发育和应激适应至关重要.
在小麦的关键祖先Aegilops tauschii中,m6A调节网络仍然在很大程度上未被描述.

研究的目的:

在Aegilops tauschii.中系统地识别和描述m6A相关基因.
研究这些基因的基因组组织,进化特征和应激反应作用.
在小麦化过程中探索m6A基因的遗传变异,以改善作物.

主要方法:

全基因组搜索m6A相关基因 (写字器,阅读器,擦拭器).
遗传学分析,基因结构和保存域分析.
对cis作用元素的分析,表达特征 (RNA-seq),qPCR验证和重新测序数据的分析.

主要成果:

在Aegilops tauschii.中确定了25个假定的m6A基因 (5个写字,13个读字,7个擦拭字).
遗传学分析揭示了子家族内保存的基因结构和域.
基因具有与激素信号传递,生长和应激反应相关的 cis 作用元素.
表达式分析确定了应激反应的候选人,其中5个通过qPCR得到验证.
在小麦化过程中,在m6A基因中观察到显著的遗传变异和瓶,与农学特征的潜在关联.

结论:

这项研究提供了Aegilops tauschii.m6A相关基因的综合基因和进化概述.
这些发现促进了进一步的功能研究,并突出了m6A基因在小麦遗传改进方面的潜力.
了解m6A基因进化为作物化和适应策略提供了洞察力.

关键词:

艾吉洛普斯 (Aegilops tauschiichii) 是一个的动物.表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式表达方式遗传变异遗传变异遗传变异 m6A 一个很好的.

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