宿主细胞对罗塔病毒感染的反应,重点是病毒 - 葡萄糖相互作用,胆固醇代谢和天生的免疫力
Molly Raque1, Sergei A Raev1, Yusheng Guo1
1Center for Food Animal Health Research Program, Department of Veterinary Preventive Medicine, College of Veterinary Medicine, Department of Animal Sciences, College of Food Agricultural and Environmental Sciences, The Ohio State University, Wooster, OH 43210, USA.
Viruses
|July 29, 2023
概括
轮状病毒A (RVA) 菌株G9P[13]和G5P[7]表现出不同的复制机制. 与G5P不同的是,G9P[13]显著改变宿主基因表达,免疫反应和代谢途径[7].
科学领域:
- 病毒学和宿主-病原体相互作用
- 分子生物学和基因组学
- 胃肠道学和免疫学
背景情况:
- 罗塔病毒A (RVA) 是儿童和动物急性病毒性胃肠炎的主要原因.
- 了解RVA复制和发病因子对于开发控制策略至关重要.
- 以前的研究表明,酸去除对RVA菌株G9P[13]和G5P[7]复制的差异性影响.
研究的目的:
- 为了比较主体转录基因组对猪内肠 (PIEs) 对RVA-G9P[13]和RVA-G5P[7]感染的反应.
- 研究这些RVA菌株对免疫反应,胆固醇代谢和宿主细胞附着的差异性影响.
- 阐明细胞对新兴/重新出现和历史RVA菌株的反应的独特机制.
主要方法:
- 用RVA-G9P[13]和RVA-G5P[7],感染猪阴道内肠 (PIEs) 的情况.
- 转录组分析以评估全球基因表达变化.
- 专注于参与免疫反应,胆固醇代谢和糖系转移酶的基因.
主要成果:
- 与RVA-G5P[7] (488个基因) 相比,RVA-G9P[13]感染诱导了显著更大的基因表达调制 (4093个基因).
- 免疫信号通路的微分调节:G9P[13]上调调节,而G5P[7]则抑制它们.
- 这两种菌株都影响了胆固醇转运基因;G9P[13]独特地影响了胺合成途径.
结论:
- RVA菌株G9P[13]和G5P[7]表现出不同的宿主-病原体相互作用机制.
- G9P[13]对宿主基因表达,免疫信号和代谢途径产生更深远的影响,包括胺合成.
- 这些发现突出了RVA受体相互作用和宿主反应的关键差异,这对于设计有效的RVA控制策略至关重要.
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