结块性皮肤疾病病毒基因组序列分析:假定空间时间流行病学,单基因与全基因组分化和基因组进化
Floris C Breman1, Andy Haegeman1, Nina Krešić1
1Sciensano, Unit Exotic and Vector Borne Diseases (ExoVec), Groesselenberg 99, B-2800 Ukkel, Belgium.
Viruses
|July 29, 2023
概括
全基因组测序揭示了在亚洲传播的块性皮肤病病毒 (LSDV) 菌株. 全基因组测序 (WGS) 提供了比单基因测序更好的分辨率,以了解LSDV的演变和传播.
科学领域:
- 兽医病毒学 兽医病毒学
- 分子流行病学分子流行病学
- 在 Poxviridae 的研究中.
背景情况:
- 块状皮肤病病毒 (LSDV) 在牲畜中造成重大经济损失.
- 目前在亚洲LSDV的扩散需要了解其遗传学关系和基因组进化.
- 关于亚洲各地LSDV菌株的详细遗传多样性和进化模式的知识有限.
研究的目的:
- 为了重建结块性皮肤病病毒 (LSDV) 菌株的全基因组基因组.
- 为了比较全基因组测序 (WGS) 基因组与基于单基因的基因组.
- 分析LSDV进化和分散的种群和时空模式.
主要方法:
- 使用全基因组测序 (WGS) 来重建LSDV的基因组.
- 进行了家族遗传学分析,并与基于单基因的家族遗传进行了比较.
- 网络重建被用来研究人口和时空模式.
主要成果:
- 来自1.2集群内的多个菌株的LSDV菌株与欧亚和南亚的爆发相关.
- 来自2.5集群的菌株被确定为在东南亚蔓延.
- 在LSDV基因组的5'和3'侧边区域中确定了三个高度可变的基因区域,这些区域可能参与免疫逃避.
结论:
- 与单基因测序相比,全基因组测序 (WGS) 为LSDV提供了优越的遗传学和时空分辨率.
- 建议常规使用WGS来准确的LSDV菌株特征和疫情调查.
- 鉴定到的可变基因区域为未来对LSDV进化和免疫逃避机制的研究提供了潜在的目标.
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