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Highly Sensitive Assay for Measurement of Arenavirus-cell Attachment
Published on: March 2, 2016
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带有小细胞外囊释放的非外鸟类转录病毒具有高度传染性.
Zuopei Wang1, Menghan He1, Han He2
1Laboratory of Molecular Virology and Immunology, Technology Innovation Center, Haid Research Institute, Guangdong Haid Group Co., Ltd., Panyu, Guangzhou 511400, China.
Viruses
|July 29, 2023
概括
鸟类骨髓病毒 (ARVs) 可以在细胞外囊泡 (EVs) 中释放,这可能会增强它们的感染力和传播. 这些EV可能会导致家禽的ARV毒性.
科学领域:
- 病毒学 病毒学
- 细胞生物学 细胞生物学
- 免疫学 免疫学 免疫学
背景情况:
- 鸟类骨病毒 (ARV) 在家禽中引起重大疾病.
- 抗病毒药物的输出和传播机制,特别是它们对细胞外囊泡 (EVs) 的潜在使用,尚不清楚.
- 囊泡封装的病毒可以逃避免疫检测并增强系统性传播.
研究的目的:
- 调查ARV是否利用细胞囊泡贩运进行出口和细胞间传播.
- 描述ARV感染细胞产生的细胞外囊泡.
- 确定这些EV在ARV感染性和毒性中的作用.
主要方法:
- 传输电子显微镜 (TEM) 和冷电子断层扫描 (Cryo-EM) 用于可视化囊泡和病毒颗粒.
- OptiPrep密度梯度超离心法以隔离细胞外囊泡.
- 西方涂抹检测EV中的病毒蛋白质.
- 传染性测试比较单独的病毒和混合EVs的病毒的传染性.
主要成果:
- 被ARV感染的长细胞产生了小 (~100 nm) 细胞外囊泡 (EV).
- 克里奥-EM证实,这些EV中不含完整的ARV病毒,但确实含有病毒蛋白,包括融合性p10 FAST蛋白.
- 含有ARV成分的EV显著增加了混合病毒颗粒的传染性 (高达50倍).
结论:
- 抗病毒药物可以从细胞外囊中感染的细胞中释放出来.
- 这些与ARV相关的EV,可能携带融合性蛋白质,可能在增强病毒毒性和传播方面发挥作用.
- 细胞外囊泡代表ARV免疫逃避和全身传播的潜在机制.
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