相关实验视频

Updated: Jul 20, 2025

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Direct Agroinoculation of Maize Seedlings by Injection with Recombinant Foxtail Mosaic Virus and Sugarcane Mosaic Virus Infectious Clones

Published on: February 27, 2021

番茄马赛克病毒在中国北部三个省份的基因变异性和分子进化

Jinfu Lyu¹, Yuanyuan Yang¹, Xiaohui Sun²

¹Shandong Provincial University Laboratory for Protected Horticulture, Shandong Facility Horticulture Bioengineering Research Center, Weifang University of Science and Technology, Shouguang 262700, China.

|July 29, 2023

概括

番茄马赛克病毒 (ToMV) 在中国北部广泛传播,菌株之间存在显著的遗传变异性. 净化和选择压力正在稳定ToMV种群,使其成为一个统一的菌株.

科学领域:

植物病毒学植物病毒学
分子进化是分子进化的过程.
农业科学农业科学

背景情况:

像番茄马赛克病毒 (ToMV) 这样的RNA病毒通过突变表现出关键的适应和进化.
ToMV是一种具有经济意义的多巴莫病毒,影响着全球番茄产量.
了解中国的ToMV遗传多样性对于疾病管理至关重要.

研究的目的:

研究中国北部番茄马赛克病毒 (ToMV) 的遗传多样性和种群结构.
分析ToMV群体内的单核酸多态 (SNPs) 和全基因组变异.
提供对ToMV演变的见解,并为疾病控制策略提供信息.

主要方法:

2016年从中国北部省份收集了425个番茄样本.
使用逆转录PCR (RT-PCR) 检测ToMV.
对ToMV单核酸多态 (SNPs) 和对对序列身份的分析.

主要成果:

在所有采样区域中检测到ToMV的高发病率 (67.15%).
ToMV基因组显示出具有不均变异的进化保护.
在ToMV菌株中观察到显著的基因组变异性 (73.2-99.6%的核酸同一性).
纯化和选择作用于ToMV群体的证据.

结论:

由于选择压力,中国北部的ToMV种群正在稳定为统一的菌株.
监测流行的ToMV菌株及其毒性对于有效的预防和控制在中国至关重要.
这些发现有助于理解病毒演变和在农业环境中管理ToMV.

关键词:

ToMV 菌株变异的变化遗传多样性遗传多样性遗传学分析遗传学分析番茄马赛克病毒病毒

更多相关视频

Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

Published on: February 8, 2020

Fluorescence in situ Hybridizations FISH for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Fluorescence in situ Hybridizations FISH for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Published on: February 24, 2014

相关概念视频

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...

Gene Flow

Gene Flow

Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.

Viral Mutations

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...