在调节循环时钟方面,Synechococcus elongatusRNA结合蛋白Rbp2的作用
Briana M McKnight1,2, Shannon Kang1,2, Tam H Le3
1Department of Molecular Biology, University of California, San Diego, La Jolla, CA, USA.
Journal of biological rhythms
|July 29, 2023
概括
蓝藻细菌的昼夜时间表.
科学领域:
- * 分子生物学 * 分子生物学
- * * 时间生物学
- * 细胞生物学 * 细胞生物学
背景情况:
- * 蓝藻细菌的昼夜时钟依赖于KaiA,KaiB和KaiC蛋白质振荡器.
- *在体内,KaiA和KaiC蛋白质表现出动态的亚细胞定位,在白天和夜晚之间从扩散到极地定位转移.
- *了解调节这种局部化的机制对于理解昼夜时钟的功能至关重要.
研究的目的:
- * 调查KaiC的CI域在其极地定位中的作用.
- * 识别不同局部状态下与KaiC相互作用的蛋白质.
- *阐明Rbp2在昼夜时钟调节和KaiC定位中的作用.
主要方法:
- *在体外生化测试以评估KaiC的CI域功能.
- *蛋白质组分析以确定与KaiC相关的蛋白质.
- * 基因操纵 (例如,基因删除) 来研究Rbp2.2的作用.
- * 对昼夜节律和蛋白质定位的表型分析.
主要成果:
- * 六米 KaiC 的 CI 域足以用于极地准.
- *增加的KaiC ATPase活性与增强的极地定位相关.
- *Rbp2是一种RNA结合蛋白,与局部KaiC相关.
- *Rbp2的损失会损害KaiC的局部化,并导致长期的昼夜表型.
- * Rbp2的RNA结合活性对于时钟功能至关重要.
结论:
- *KaiC的CI域和ATPase活性是其极地定位的关键调节者.
- *Rbp2通过影响KaiC局部化,在昼夜时钟调节中发挥着至关重要的,以前未知的作用.
- *通过Rbp2促进KaiC的适当亚细胞局部化,对于精确的体内昼夜时钟至关重要.
相关概念视频
Circadian Rhythms and Gene Regulation
4.1K
The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent...
4.1K
Bacterial RNA Polymerase
29.7K
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
29.7K
Transcriptional Regulation: Riboswitches
61
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
61
Eukaryotic RNA Polymerases
24.3K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
24.3K
Translational Regulation
45
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
45
Transcription Initiation
16.5K
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
16.5K


