相关实验视频
Updated: Jul 20, 2025

07:23
Improved Rodent Model of Myocardial Ischemia and Reperfusion Injury
Published on: March 7, 2022
6.0K
锡尔图因5通过调节线粒体顺和氧化应激减轻肝脏缺血/再输损伤
Yan Hu1,2, Xinyao Tian3,4, Yan Zhao1
1Department of Pharmacology, Dalian Medical University, Dalian, China.
Antioxidants & redox signaling
|July 29, 2023
概括
赛尔图因5 (SIRT5) 通过减少线粒体氧化应激,保护肝脏免受缺血/反 (I/R) 损伤. 这项研究揭示了SIRT5脱化PRDX3,缓解损伤并提供了一种新的治疗策略.
科学领域:
- 生物化学 生物化学
- 细胞生物学 细胞生物学
- 医学科学 医学科学 医学科学
背景情况:
- 线粒体功能障碍驱动肝脏缺血/再生 (I/R) 损伤.
- Sirtuin 5 (SIRT5) 是一种 lysine desuccinylase,调节线粒体功能,但其在肝脏I/R中的作用尚不清楚.
- 在I/R损伤期间观察到线粒体化增加.
研究的目的:
- 研究SIRT5在肝脏I/R损伤中的作用和机制.
- 为了确定SIRT5是否表现出对I/R诱导损伤的肝脏保护作用.
- 在这种情况下,阐明SIRT5调节的分子点和途径.
主要方法:
- 已建立的小鼠模型的肝脏I / R与SIRT5抑制和SIRT5过度表达的条件.
- 在I/R模型和患者样本中分析了线粒体化水平和SIRT5表达.
- 使用生物化学测定研究了SIRT5和PRDX3之间的相互作用和功能关系.
主要成果:
- 在肝脏I/R模型和人肝移植患者中,SIRT5水平下降.
- SIRT5过度表达减弱了肝脏I/R损伤,减少了氧化应激,亡和炎症.
- 在K84处SIRT5脱化PRDX3,增强其抗氧化功能并减轻线粒体的氧化应激.
结论:
- 在肝脏I/R损伤时,SIRT5起着至关重要的肝脏保护作用.
- 该机制涉及SIRT5介导的PRDX3脱化,这可以对抗线粒体的氧化应激.
- 准SIRT5为预防肝脏I/R损伤提供了一个新的治疗策略.
相关概念视频
Electron Transport Chain: Complex I and II
14.5K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
14.5K
Regulation of the Unfolded Protein Response
2.5K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.5K
Peroxisomes
13.6K
Peroxisomes are specialized organelles present in fungi, plant, and animal cells. It can vary in number, size, morphology, and activity depending on the type of tissue and the nutritional state of the cell. For example, cells with active lipid metabolism, such as adipocytes, neurons, and hepatocytes, have more peroxisomes than other cells in the body. Besides their primary role in breaking down complex organic molecules, peroxisomes can also synthesize specific macromolecules and participate in...
13.6K
Liver Regeneration
3.3K
The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
3.3K

