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Updated: Jul 20, 2025

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N-终端的LysSN-His-tag改善了Bacillus subtilis中细胞内复合蛋白的产生

Ngan Thi Phuong Le1,2, Trang Thi Phuong Phan1,2,3, Tuom Thi Tinh Truong1,2,4

  • 1Center for Bioscience and Biotechnology, University of Science, Ho Chi Minh City, Vietnam.

Cell biochemistry and function
|July 29, 2023
PubMed
概括

一个新型的融合标签,LysSN-xHis,增强了Bacillus subtilis中的重组蛋白质生产. 这个标签显著增强了像EGFP这样的低表达蛋白质,并改善了像BgaB和GFP+这样的高表达蛋白质.

关键词:
这种细菌是 Bacillus subtilis.这就是 LysSN.LysSN-他的他的在N端的核聚变中.在Pgrac的推广者.Pgrac212 的发起人是Pgrac212 的发起人.的 pHT 矢量.

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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