基胺在暴露于人类质母细胞上皮细胞 (LN-229) 时引发的细胞后果
Kizhakkepurakkal B Megha1, Parayanthala V Mohanan1
1Toxicology Division, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology (Govt. of India), Poojapura, Trivandrum, Kerala, India.
Journal of biochemical and molecular toxicology
|July 29, 2023
概括
长期使用胺,特别是作为抗抑郁药或娱乐药物,可能会导致显著的细胞损伤. 这项研究揭示了胺.
科学领域:
- 神经科学是一个神经科学.
- 药理学 药理学是指药理学的学科.
- 细胞生物学 细胞生物学
背景情况:
- 胺,一种N-甲基-D-酸盐 (NMDA) 受体对抗剂,已被批准用于麻醉,但也用于治疗疼痛,抑郁和止痛.
- 人们对胺的长期安全性存在担忧,特别是在抗抑郁药和娱乐用途中.
- 了解凯胺在麻醉之外的细胞效应对于评估其治疗风险至关重要.
研究的目的:
- 为了研究胺基因在LN-229细胞上的体外细胞毒性.
- 评估胺对细胞活力和完整性的剂量依赖性影响.
- 为了确定胺诱导的细胞死亡和损伤的特定标记物.
主要方法:
- 暴露LN-229细胞不同剂量的胺.
- 细胞活力测试以确定剂量依赖的反应.
- 微观分析使用Giemsa,Rhodamine phalloidin,DAPI和Acridine的色染色来评估形态和核变化.
- 测量乳酸脱酶泄漏和互白素-1β (IL-1β) 的释放.
主要成果:
- 胺对LN-229细胞表现出剂量依赖的细胞毒性作用.
- 观察到细胞形态和核完整性的显著变化.
- 核凝结,乳酸脱酶泄漏和增加IL-1β释放表明了细胞死亡.
- 胺暴露抑制了细胞迁移.
结论:
- 长期暴露于非麻醉剂的胺可以诱导显著的体外细胞毒性.
- 胺的副作用包括细胞亡,细胞功能受损和炎症.
- 这些发现凸显了与非麻醉性指示的慢性胺使用相关的潜在风险.
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