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在Capsicum物种的刺痛性Pun1位点中识别新型SNP

Ariza Gulzar¹, Ajaz Malik², Geetika Malik³

¹Division of Vegetable Science, Faculty of Horticulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Srinagar, Jammu & Kashmir, India.

Molecular biology reports

|July 29, 2023

概括

分子分析揭示了遗传变异,包括PunI基因中的删除和SNP,解释了Capsicum基因型之间的囊素度和度的差异. 蛋白质结构的变化会影响素的产生.

科学领域:

植物遗传学植物遗传学
分子生物学分子生物学
农业科学农业科学

背景情况:

素和素类化合物是决定Capsicum物种的刺痛性的关键化合物.
来自西北喜马拉雅山脉和各种印度陆地品种的子生殖质被描述.
专注于了解不同Capsicum基因型中尖性的分子变异.

研究的目的:

根据素度来表征的生殖质.
为了阐明高,中,和非刺痛的Capsicum物种之间刺痛性的分子变异.
为了确定与素生产和敏性相关的遗传标记.

主要方法:

分析了49种的基因型,包括繁殖线和商业陆地品种.
在基因型中量化素含量.
对PunI基因的分子分析,包括DNA片段放大,测序和SNP发现.
在非的品种中对遗传删除的调查.

主要成果:

观察到素含量的显著变化,其中Bhut Jolokia (Capsicum chinense) 的度最高 (10,500.75μg/g).
在非敏的Nishat-1 (Capsicum annuum var. 在AT3的促进子和第一个外形中发现了2.5 kb的删除. 总量 (英语:grossum) 是指总量的总量.
在尖品种的PunI基因中确定了单核酸多态 (SNP),与氨基酸和蛋白质结构变异相关.

结论:

观察到的蛋白质结构变异可能是基因型之间的不同素生产的原因.
蛋白质构成影响基质相互作用,从而影响素合成.
遗传变异,包括删除和SNP,是Capsicum物种尖性的关键决定因素.

关键词:

氨基酸变异的变化卡普赛辛是一种素.这就是Capsicum Capsicum.打字 1 打字 1 打字 1 打字刺痛的刺痛性刺痛性的刺痛性在SNP中,SNP是SNP.序列化是指测序的使用.维生素C 维生素C是一种

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