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来自组织模拟系统的介质干细胞细胞外囊增强通过形成迁移性细胞板的表皮再生
Jacob G Hodge1,2, Jennifer L Robinson1,3,4,5,6, Adam J Mellott7,8
1Bioengineering Graduate Program, University of Kansas, Lawrence, KS, USA.
Tissue engineering and regenerative medicine
|July 29, 2023
概括
一个新的3D培养系统增强了脂肪衍生干细胞 (ASC) 机密体,促进了表皮再生. 来自3D培养的细胞外囊 (EVs) 促进了角质细胞的迁移和分层,与来自2D培养的细胞不同.
科学领域:
- 再生医学是一种再生医学.
- 干细胞生物学 干细胞生物学
- 组织工程是组织工程.
背景情况:
- 脂肪酸衍生干细胞 (ASCs) 分泌的因素对于伤口愈合和表皮再生至关重要.
- 传统的二维细胞培养改变了ASC的秘密动态,在理解它们的再生潜力方面产生了模两可.
- 识别特定的ASC秘密组分驱动状细胞上皮质化对于治疗开发至关重要.
研究的目的:
- 研究一种新型3D水凝系统对ASC秘密组合和再生能力的影响.
- 为了比较2D和3D培养环境之间的ASC秘密中的功能差异.
- 评估ASC分泌体内细胞外囊泡 (EVs) 在调节角质细胞行为中的作用.
主要方法:
- 利用组织模拟的3D水凝系统培养ASC并保持再生的表型.
- 按分子量和EVs的存在/缺席分层了ASC秘密体.
- 评估了不同分泌组分的能力,以增强角质细胞的迁移,增殖,分化和形态.
主要成果:
- 与二维培养相比,3D培养显著提高了蛋白质分泌 (>100 kDa 分数) 约50%,比2D培养显著提高了50%.
- 3D ASC 分泌的>100 kDa 分数最大限度地改善了角质细胞上皮质化功能.
- 3D ASC衍生的EV显著促进了角质细胞分层,集体迁移和介质细胞过渡,而2D EV显示出反迁移效应.
结论:
- 培养环境极大地影响ASC秘密体的再生能力.
- 在3D系统中培养的ASC产生了具有卓越伤口愈合和表皮再生潜力的秘密体.
- 来自ASC的EV是表皮再生的关键媒介,突出显示了它们对未来临床应用的潜力.
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