群A型链球菌离子扩散促进蛋白通过调节细胞内金属度,有助于免疫逃避
Chihiro Aikawa1, Akihide Shimizu1, Makoto Nakakido2
1Department of Microbiology, Graduate School of Medicine, Kyoto University, Kyoto, 606-8501, Japan.
Biochemical and biophysical research communications
|July 29, 2023
概括
菌根杆菌利用MntE和CzcD载体来管理和,这对细菌的生存和毒性至关重要. 破坏这些载体会削弱病原体,影响宿主防御和蛋白酶活动.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 病原发生和发病的过程.
背景情况:
- 阴离子扩散促进剂 (CDF) 对金属稳态和细菌病原体中毒性至关重要.
- 菌 (A组菌,GAS) 采用MntE和CzcD CDF载体,分别用于 (Mn) 和 (Zn) 的运输.
研究的目的:
- 研究MntE和CzcD在GAS病毒性和宿主病原体相互作用中的作用.
- 阐明Mn和Zn稳态对细菌生存和毒性因子活性的影响.
主要方法:
- 删除突变发生,以产生缺乏MntE和CzcD的GAS菌株.
- 在体外测定使用巨分化的THP-1细胞和中性粒细胞.
- 在体内小鼠感染模型.
- 链球菌囊蛋白酶 (SpeB) 活性和成熟的分析.
主要成果:
- 缺乏MntE和CzcD的GAS菌株在宿主免疫细胞中的活力降低,感染小鼠的存活率增加.
- 缺乏MntE影响了与Mn水平相关的依赖PerR的氧化应激反应.
- 和在缺乏菌株中抑制了毒性因子SpeB的成熟和蛋白质分解活性.
结论:
- MntE和CzcD对于GAS毒性至关重要,有助于金属耐药性和病原体生存.
- 通过MntE和CzcD维持细胞内Mn和Zn水平对于GAS致病性至关重要,影响宿主免疫逃避和毒性因子功能.
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