在城市水系统中应用高通量测序技术和用于病原体检测的分析工具:进展和未来前景
Yanmei Zhao1, Fang Huang2, Wenxiu Wang3
1State Key Laboratory of Urban Water Resource and Environment, School of Civil & Environmental Engineering, Harbin Institute of Technology Shenzhen, Shenzhen 518055, China.
The Science of the total environment
|July 29, 2023
概括
高通量测序提供了城市水源病原体的先进检测,改善了公共卫生. 本次审查强调了新技术和生物信息学,以提高水安全和病原体监测.
科学领域:
- 环境微生物学环境微生物学
- 公共卫生 公共卫生
- 分析水质,分析水质.
背景情况:
- 城市用水系统是各种病原性微生物的息地,对公共卫生构成重大风险,并导致水传播疾病.
- 传统的病原体检测方法受到长时间的测试时间,高的识别值和狭窄的适用性限制.
- 环境样本的复杂性和多样性挑战了对微生物污染物的有效监测.
研究的目的:
- 审查高通量测序技术的现状,以识别城市水系统中的致病微生物.
- 总结一下新型技术,使复杂的环境矩阵中的微生物病原体的全谱检测.
- 讨论城市水系统中病原体研究和生物安全的未来前景.
主要方法:
- 对用于病原体识别的高通量测序技术的审查.
- 分析传统检测方法的局限性.
- 编译病原体分析软件,平台和数据库.
- 讨论综合测序和生物信息学方法.
主要成果:
- 高通量测序能够在复杂的城市水样中全面检测微生物的痕迹.
- 新技术克服了传统方法在速度,灵敏度和范围方面的局限性.
- 为实际应用提供精准的病原体分析工具的精选列表.
- 将目标/非目标和短/长读序列与生物信息学相结合,提高了病原体检测能力.
结论:
- 高通量测序在检测城市水源病原体方面取得了重大进展.
- 未来的研究需要准确,敏感的检测方法,标准化的程序和强大的生物信息学支持.
- 综合测序和生物信息学方法对于改善城市水系统的生物安全至关重要.
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