来自Listeria monocytogenes的膜囊泡可能是潜在的抗原递送载体
Mingyuan Tang1, Sicheng Tian1, Kehan Chen1
1Department of Public Health Laboratory Sciences, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610061, China.
International journal of pharmaceutics
|July 29, 2023
概括
李斯特菌单细胞原体膜囊泡 (MVs) 是安全的免疫原体,作为有效的 humoral 免疫辅助剂. 这些MV显示出作为抗原和药物的新型输送载体的潜力.
科学领域:
- 细菌学 细菌学是一门学科.
- 免疫学 免疫学 免疫学
- 纳米技术 纳米技术
背景情况:
- 来自Listeria monocytogenes (LM) 的膜囊泡 (MVs) 是纳米级的,含有细菌成分.
- 目前正在调查LM MVs作为潜在的新型交付载体.
- LM MVs的安全性和免疫性需要进行体内评估.
研究的目的:
- 在体外和体内评估LMMVs的安全性和免疫性.
- 确定LM MVs作为抗原和药物输送载体的潜力.
- 为了评估LM MVs的辅助性质.
主要方法:
- 隔离LMM的MVs. 这是一个很好的方法.
- 用LM MVs进行RAW264.7和DC2.4细胞的体外刺激.
- 通过C57BL/6小鼠的腹腔内注射进行体内安全性评估.
- 在体内通过腹膜内免疫和与卵泡素的皮下联合注射进行免疫性检测.
主要成果:
- 在免疫细胞中,LM MVs诱导炎症性细胞因子 (IL-1β,TNF-α,IL-6,IL-10) 的分泌.
- 肌内注射LM MVs在小鼠中没有致命效应或器官损伤,证实了安全性.
- 在免疫接种后,LM MVs诱导了高水平的LM MV特定的IgG抗体.
- 在与卵胺联合注射时,LM MVs表现出与类似的幽默免疫辅助效应.
结论:
- LM MVs具有良好的安全性和有效的免疫性.
- LM MVs可以作为幽默性免疫辅助剂发挥作用.
- LM MVs代表了对抗原和药物输送载体的有希望的新选择.
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