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转基因-CSF通过ERK1/2-依赖的途径明显影响人类单细胞和巨细胞

Negar Farzam-Kia¹, Ana Carmena Moratalla¹, Florent Lemaître¹

¹Department of Neurosciences, Faculty of Medicine, Université de Montréal, QC, Canada; Centre de Recherche du Centre Hospitalier de l'Université de Montréal (CRCHUM), Montreal, QC, Canada.

Immunology letters

|July 29, 2023

概括

颗粒细胞-巨细胞殖民地刺激因子 (GM-CSF) 不同地影响人类单细胞和巨细胞. 转基因-CSF影响免疫媒介表达和信号通路,其中一些影响依赖于ERK.

科学领域:

免疫学免疫学免疫学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

人体单细胞和巨细胞是免疫和组织恒温的关键髓状细胞.
颗粒细胞-巨细胞殖民地刺激因子 (GM-CSF) 对于髓状细胞的发育和功能至关重要.

研究的目的:

为了比较GM-CSF对人类单细胞和单细胞衍生的巨细胞免疫媒介表达的影响.
研究信号通路,特别是ERK1/2在GM-CSF诱导反应中的作用.

主要方法:

隔离人类单细胞并将其分化为巨细胞.
用GM-CSF刺激的细胞.
分析了表面标记物表达 (CD80,ICAM-1,HLA-DR,IL-15) 和细胞因子分泌 (IL-4,IL-6,TNF,CXCL10,IL-27).
研究了信号通路 (ERK1/2,STAT3,STAT5,SAPK/JNK),并使用U0126来评估ERK1/2的参与.

主要成果:

在两种细胞类型中,GM-CSF同样调节了CD80,ICAM-1和HLA-DR.
转基因-CSF对表面IL-15的表达有不同的影响,在巨细胞中增加它,但在单细胞中减少它.
转基因-CSF改变了细胞因子分泌特征,提高了巨细胞中的几个介质的调节,同时降低了单细胞中的其他介质.
在两种细胞类型中,GM-CSF激活了ERK1/2,STAT3,STAT5和SAPK/JNK通路.
ERK1/2通路与GM-CSF对IL-15表达和细胞因子分泌的差异性影响有关,部分与表面标记物调节有关.

结论:

在人类单细胞和巨细胞中,GM-CSF诱导了不同的反应.
该ERK1/2信号通路在调解GM-CSF对这些髓状细胞的影响方面发挥着重要的,尽管差异性的作用.

关键词:

化学物质化学物质是化学物质.细胞因子细胞因子甲基因激活蛋白激酶 (MAPK) 是一种中基因激活蛋白激酶.骨髓状细胞细胞是什么?信号传输信号传输

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