相关实验视频
Updated: Jul 31, 2026
08:31
Porous Silicon Microparticles for Delivery of siRNA Therapeutics
Published on: January 15, 2015
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在水系统中具有不同PEG含量的siRNA载脂纳米颗粒的生物物理特征
Ki Hyun Kim1, Ripesh Bhujel1, Ravi Maharjan1
1BK21 FOUR Team and Integrated Research Institute for Drug Development, College of Pharmacy, Dongguk University, Gyeonggi 10326, Republic of Korea.
概括
较高的PEGylation增强了对环境压力因素的脂质纳米粒子 (LNP) 稳定性. 控制PEG度对于在各种条件下保持LNP和siRNA稳定性至关重要.
科学领域:
- 制药纳米技术 制药纳米技术
- 生物物理化学 生物物理化学
背景情况:
- 脂质纳米颗粒 (LNP) 是药品成分的有希望的载体.
- 在环境压力因素下的LNP稳定性对于药物输送应用至关重要.
研究的目的:
- 为了研究PEGylation度对siRNA载荷的LNP稳定性的影响.
- 评估LNP对物理化学压力因素的弹性,如振动,温度和冷-解周期.
主要方法:
- 使用动态光散射 (DLS),流量成像显微镜 (FIM) 和离子交换色谱 (IEX).
- 应用端对端的动,升高的温度和重复的冷解周期作为压力条件.
- 评估了在表面上具有不同PEG度 (0.5%和1.0%) 的LNP.
主要成果:
- 与0.5%的PEG-LNP相比,1.0%的PEG-LNP表现出更高的合稳定性,特别是在高温和多次冷解周期下.
- 0.5%的PEG-LNP仅在低pH或盐下表现出聚合阻力,而1.0%的PEG-LNP在pH4以外保持稳定.
- 在所有测试的压力条件下,1.0%的PEG-LNP表现出增强的siRNA稳定性.
结论:
- 增加的PEGylation (1.0%) 显著改善了LNP体和siRNA对环境压力因素的稳定性.
- 仔细控制PEG度对于优化LNP稳定性和确保有效的siRNA传递至关重要.
- 了解压力下的LNP不稳定机制对于开发强大的LNP配方至关重要.
相关概念视频
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
02:30siRNA - Small Interfering RNAs
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
02:57piRNA - Piwi-interacting RNAs
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
02:15Experimental RNAi
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
02:30Small interfering RNAs (siRNA)
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...