铜接触面的抗病毒活性对MS2胆固醇和肝炎a病毒的抗病毒作用
Juan C Camacho1, Y Carol Shieh1, Benjamin W Redan1
1Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, 6502 S. Archer Rd, Bedford Park, IL 60501, USA.
Journal of applied microbiology
|July 29, 2023
概括
铜表面有效地使MS2菌无活化,特别是在酸性条件和简单的介质中. 然而,MS2可能不能准确地代表铜上的甲型肝炎病毒 (HAV) 失活.
科学领域:
- 食品安全 食品安全
- 微生物学 微生物学
- 材料科学是一种材料科学.
背景情况:
- 从非食品接触面到食品的病毒交叉污染是一个重大的公共卫生问题.
- 了解病毒与金属的相互作用对于制定有效的减轻传播风险的策略至关重要.
研究的目的:
- 为了研究MS2菌体和甲型肝炎病毒 (HAV) 在金属表面,特别是铜表面的无活化.
- 评估影响铜病毒失活的因素,包括环境条件和媒介成分.
- 评估MS2在铜无活化研究中作为HAV的替代品的适用性.
主要方法:
- MS2菌体和HAV暴露于99.9%的铜表面.
- 在各种条件下 (pH,介质组成,食物矩阵) 测量了病毒稳定性和无活化率.
- 用FRhK-4细胞感染性测试来确定HAV存活率.
主要成果:
- 在不钢上MS2是稳定的,但在铜上被禁用 (0.95 log10 PFU min-1).
- 与富含蛋白质介质相比,铜在酸性 (pH3.5) 和简单介质 (PBS) 中显示出更大的MS2无活化.
- 在测试条件下,HAV在铜的生存时间比MS2更长 (0.17 log10 PFU min-1减少).
结论:
- 铜表面增强病毒失活,特别是在酸性环境和简单的溶液中.
- 铜对病毒失活的有效性受周围环境的影响.
- 在评估铜表面的病毒无活化时,MS2可能不是HAV的可靠替代品.
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