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Updated: Jul 20, 2025

A Colorimetric Assay that Specifically Measures Granzyme B Proteolytic Activity: Hydrolysis of Boc-Ala-Ala-Asp-S-Bzl
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通过使用光局部化报告器,分析活细胞内蛋白酶酶B和酶-8的活性

Nora Bruning1, Vivian Bönnemann1, Carsten Watzl1

  • 1Department of Immunology, Leibniz Research Centre for Working Environment and Human Factors at TU Dortmund (IfADo), Dortmund, Germany.

Methods in cell biology
|July 29, 2023
PubMed
概括

这项研究引入了一种使用光记者来追踪活细胞中granzyme B和caspase-8活动的新方法,有助于研究自然杀手细胞的细胞毒性功能.

关键词:
细胞灭亡 (apoptosis) 是一种死亡的过程.卡斯帕斯海域的卡斯帕斯海域细胞细胞毒性细胞毒性大酵素 (Granzyme) 是一种酶.自然杀手细胞自然杀手细胞

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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