相关实验视频

Updated: Jul 20, 2025

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress

Published on: October 11, 2024

哺乳动物4E-BPs的功能类型揭示了TOR在全球植物翻译中的作用

Yihan Dong¹, Ola Srour¹, Nina Lukhovitskaya¹

¹Institut de biologie moléculaire des plantes UPR2357 du CNRS, Université de Strasbourg, Strasbourg, France.

|July 30, 2023

概括

植物利用独特的4E结合蛋白 (4EBPs) 来控制蛋白质合成. 拉巴胺素 (TOR) 信号传递的机械标使这些非典型的4EBP失活,调节翻译启动和植物发育.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
生物化学生化学

背景情况:

拉巴胺素的机械标 (mTOR) 通过eIF4E结合蛋白 (4E-BPs) 调节哺乳动物中的蛋白质合成.
在植物中控制上限依赖转化启动的TOR的作用以前是未知的.
4E-BPs被认为是绝对动物的专属.

研究的目的:

调查拉巴胺素 (TOR) 标控制植物蛋白质合成的机制.
识别哺乳动物4E结合蛋白 (4E-BPs) 的植物同类并阐明它们的功能.
了解Arabidopsis.在翻译启动的TOR介导调节.

主要方法:

鉴定和表征阿拉比多普西斯4E-BP类蛋白质 (4EBP1/4EBP2).
对eIF4E结合基因和TOR酸化位点的分析.
在TOR激活时调查4EBP酸化状态和定位.
评估4EBP过度表达对基因表达和幼苗发育的影响.
检查TOR信号和4EBP活动之间的相互作用.

主要成果:

阿拉比多普西斯具有非典型的4E-BP类蛋白质 (4EBP1/4EBP2),它们与eIF4Es结合并抑制翻译.
通过TOR的酸化使4EBPs失活,可能是通过转移到核糖体.
4EBP2调节细胞循环基因 (CycB1;1,CycD1;1),而4EBP1影响叶绿素生物合成.
过度表达4EBP2会破坏幼苗的发育,这种发育可以通过糖-TOR信号来挽救.

结论:

在植物中,TOR通过非典型的4EBPs的失活来调节上限依赖的翻译启动.
这种机制提供了营养可用性,TOR信号和植物蛋白质合成之间的联系.
非典型的4EBP代表了在真核生物中保存的,但又独特的转化控制模式.

关键词:

CP: 植物植物在上限依赖的翻译启动.对于TOR的酸化目标,TOR的酸化目标是叶绿素生物合成叶绿素生物合成环林B 环林B 环林B 环林B 环林B 环林 D D 环林 D 环林 D 环林结合eIF4E的蛋白质.eIF4E类型的蛋白质.eIF4G类型的蛋白质.翻译控制翻译控制

更多相关视频

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Monitoring eIF4F Assembly by Measuring eIF4E-eIF4G Interaction in Live Cells

Published on: May 1, 2020

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Translating Ribosome Affinity Purification TRAP to Investigate Arabidopsis thaliana Root Development at a Cell Type-Specific Scale

Published on: May 14, 2020

相关概念视频

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

Cotranslational Protein Translocation

Cotranslational Protein Translocation

Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Transcription Attenuation in Prokaryotes

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure. Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...

Bacterial Translocation and Protein Secretion

Bacterial Translocation and Protein Secretion

Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...

Translational Regulation

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...