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通过PNGase F可访问性试验分析N链接甘氨酸的拓

Jingcheng Wang1, Jin Ye1

  • 1Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

STAR protocols
|July 30, 2023
PubMed
概括

这项研究引入了一种新的方法来确定N-甘氨酸在跨膜蛋白的位置. 它利用PNGase F酶活性来区分细胞质和光质N-甘氨酸.

关键词:
细胞生物学 细胞生物学细胞分离/细胞分离基于细胞的测试分子生物学分子生物学蛋白质生物化学 蛋白质生物化学

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